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Invitrogen
TCP1 eta Polyclonal Antibody
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FIGURE: 1 / 4
TCP1 eta Antibody (PA5-78956) in ICC/IF
Immunocytochemistry analysis of CCT7 using anti-CCT7 antibody (Product # PA5-78956) . CCT7 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-CCT7 antibody (Product # PA5-78956) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More
Product Details
PA5-78956
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human CCT7 recombinant protein (Position: Q30-D307).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell, human CACO-2 whole cell, human 293T whole cell, human HepG2 whole cell, human MCF-7 whole cellhuman HEL whole cell, human U251 whole cell, human K562 whole cell, rat C6 whole cell, mouse Neuro-2a whole cell. ICC/IF: U20S cell. Flow: HepG2 cell.
Target Information
The TCP1 eta gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CCT containing TCP-1-eta; CCT-eta; CCT7; Chaperonin containing T-complex polypeptide 1 subunit 7; chaperonin containing t-complex polypeptide 1, eta subunit; chaperonin containing t-complex subunit eta; chaperonin subunit 7 (eta); HIV-1 Nef interacting protein; HIV-1 Nef-interacting protein; MGC110985; T-complex protein 1 subunit eta; TCP-1-eta; unnamed protein product
Gene Aliases: AA408524; AL022769; CCT7; CCTETA; CCTH; Cctz; NIP7-1; TCP1ETA
UniProt ID: (Human) Q99832, (Mouse) P80313
Entrez Gene ID: (Human) 10574, (Mouse) 12468, (Rat) 297406
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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