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Invitrogen
TDG Polyclonal Antibody
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FIGURE: 1 / 15
TDG Antibody (PA5-29140) in ICC/IF
TDG Polyclonal Antibody detects TDG protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TDG protein stained by TDG Polyclonal Antibody (Product # PA5-29140) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Product Details
PA5-29140
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
C. elegans,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human TDG. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa, Jurkat, Raji, NCI-H929, TDG-transfected 293T.
Predicted reactivity: Human (99%), Mouse (88%), Rat (87%), Bovine (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mis paired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-JUN leucine zipper interactive protein JZA-3; G/T mismatch-specific thymine DNA glycosylase; hTDG; Thymine-DNA glycosylase
Gene Aliases: E130317C12Rik; hTDG; JZA-3; Jza1; TDG
UniProt ID: (Human) Q13569, (Mouse) P56581
Entrez Gene ID: (Human) 6996, (Mouse) 21665
RNA polymerase II transcription cofactor activity
damaged DNA binding
double-stranded DNA binding
uracil DNA N-glycosylase activity
protein kinase C binding
protein binding
pyrimidine-specific mismatch base pair DNA N-glycosylase activity
DNA N-glycosylase activity
mismatched DNA binding
SUMO binding
protein homodimerization activity
structure-specific DNA binding
transcription factor binding
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing N-glycosyl compounds
protein domain specific binding
DNA metabolism protein
DNA glycosylase
nucleic acid metabolism protein
negative regulation of transcription from RNA polymerase II promoter
base-excision repair
base-excision repair, AP site formation
mismatch repair
transcription, DNA-templated
embryo development
covalent chromatin modification
protein sumoylation
negative regulation of protein binding
oxidative DNA demethylation
negative regulation of chromatin binding
regulation of gene expression, epigenetic
depyrimidination
DNA demethylation
DNA repair
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
metabolic process
chromatin modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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