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Invitrogen
TDG Polyclonal Antibody
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FIGURE: 1 / 16
TDG Antibody (PA5-29140) in ICC/IF
TDG Polyclonal Antibody detects TDG protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TDG protein stained by TDG Polyclonal Antibody (Product # PA5-29140) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Product Details
PA5-29140
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human TDG. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa, Jurkat, Raji, NCI-H929, TDG-transfected 293T.
Predicted reactivity: Human (99%), Mouse (88%), Rat (87%), Bovine (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mis paired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-JUN leucine zipper interactive protein JZA-3; G/T mismatch-specific thymine DNA glycosylase; hTDG; Thymine-DNA glycosylase; unnamed protein product
Gene Aliases: E130317C12Rik; hTDG; JZA-3; Jza1; TDG
UniProt ID: (Human) Q13569, (Mouse) P56581
Entrez Gene ID: (Human) 6996, (Mouse) 21665
magnesium ion binding
mismatch base pair DNA N-glycosylase activity
nucleic acid binding
DNA binding
damaged DNA binding
double-stranded DNA binding
transcription cofactor activity
uracil DNA N-glycosylase activity
protein kinase C binding
protein binding
ATP binding
pyrimidine-specific mismatch base pair DNA N-glycosylase activity
hydrolase activity
DNA N-glycosylase activity
protein domain specific binding
mismatched DNA binding
sodium ion binding
chloride ion binding
SUMO binding
G/U mismatch-specific uracil-DNA glycosylase activity
DNA-binding transcription factor binding
G/T mismatch-specific thymine-DNA glycosylase activity
RNA polymerase II transcription cofactor activity
transcription factor binding
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing N-glycosyl compounds
protein homodimerization activity
structure-specific DNA binding
DNA metabolism protein
DNA glycosylase
nucleic acid metabolism protein
negative regulation of transcription from RNA polymerase II promoter
DNA repair
base-excision repair
base-excision repair, AP site formation
chromatin organization
cellular response to DNA damage stimulus
regulation of gene expression, epigenetic
depyrimidination
regulation of embryonic development
chromosomal 5-methylcytosine DNA demethylation, oxidation pathway
mismatch repair
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
metabolic process
embryo development
chromatin modification
negative regulation of protein binding
negative regulation of chromatin binding
DNA demethylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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