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Invitrogen
TDG Polyclonal Antibody
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FIGURE: 1 / 1
TDG Antibody (PA5-86406) in WB
Western blot analysis of TDG in Lane 1: CT-26 whole cell lysate (40 µg), Lane 2: PC12 whole cell lysate (40 µg), Lane 3: HCT116 whole cell lysate (40 µg), Lane 4: HEK293T whole cell lysate (40 µg), Lane 5: HeLa whole cell lysate (40 µg). Sample was incubated with TDG polyclonal antibody (Product # PA5-86406) using a 1:1000 dilution.
Product Details
PA5-86406
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, corresponding to amino acids 28-109 of Human TDG.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mis paired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-JUN leucine zipper interactive protein JZA-3; G/T mismatch-specific thymine DNA glycosylase; hTDG; Thymine-DNA glycosylase; unnamed protein product
Gene Aliases: E130317C12Rik; hTDG; JZA-3; Jza1; TDG
UniProt ID: (Human) Q13569, (Mouse) P56581
Entrez Gene ID: (Human) 6996, (Rat) 114521, (Mouse) 21665
magnesium ion binding
mismatch base pair DNA N-glycosylase activity
nucleic acid binding
DNA binding
damaged DNA binding
double-stranded DNA binding
transcription cofactor activity
uracil DNA N-glycosylase activity
protein kinase C binding
protein binding
ATP binding
pyrimidine-specific mismatch base pair DNA N-glycosylase activity
hydrolase activity
DNA N-glycosylase activity
protein domain specific binding
mismatched DNA binding
sodium ion binding
chloride ion binding
SUMO binding
G/U mismatch-specific uracil-DNA glycosylase activity
DNA-binding transcription factor binding
G/T mismatch-specific thymine-DNA glycosylase activity
RNA polymerase II transcription cofactor activity
transcription factor binding
hydrolase activity, hydrolyzing N-glycosyl compounds
protein homodimerization activity
structure-specific DNA binding
hydrolase activity, acting on glycosyl bonds
DNA metabolism protein
DNA glycosylase
nucleic acid metabolism protein
negative regulation of transcription from RNA polymerase II promoter
DNA repair
base-excision repair
base-excision repair, AP site formation
chromatin organization
cellular response to DNA damage stimulus
regulation of gene expression, epigenetic
depyrimidination
regulation of embryonic development
chromosomal 5-methylcytosine DNA demethylation, oxidation pathway
mismatch repair
transcription from RNA polymerase II promoter
negative regulation of protein binding
negative regulation of chromatin binding
DNA demethylation
transcription, DNA-templated
regulation of transcription, DNA-templated
metabolic process
embryo development
chromatin modification
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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