Proteintech

TDP-43 Polyclonal Antibody

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TDP-43 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of SH-SY5Y cells using 10782-2-AP (TDP-43 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Cells were fixed with 10% Formaldehyde.

TDP-43 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10782-2-AP ( TDP-43 Antibody) at dilution of 1:2000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

TDP-43 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10782-2-AP (TDP-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

TDP-43 Antibody in Western Blot (WB) — Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10782-2-AP (TARDBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

TDP-43 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug TDP-43 antibody (10782-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

TDP-43 Antibody in Immunoprecipitation (IP) — IP result of anti-TDP-43 (IP:10782-2-AP, 3ug; Detection:10782-2-AP 1:1000) with K-562 cells lysate 1000ug.

Product Details

10782-2-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2000	View 382 publications 382 publications
Immunohistochemistry (IHC)	-	View 494 publications 494 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:1,000-1:4,000	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 179 publications 179 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
ELISA (ELISA)	-	View 3 publications 3 publications
Immunoprecipitation (IP)	0.5-4.0 µg	View 26 publications 26 publications
ChIP assay (ChIP)	Assay-dependent	View 1 publication 1 publication
RNA Immunoprecipitation (RIP)	Assay-dependent	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 36 publications 36 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat, Zebrafish
Published species	Artificial Control, C. elegans, Chicken, Dog, Fruit fly, Hamster, Horse, Human, Invertebrate, Mouse, Pig, Plant, Rat, Yeast, Zebrafish
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TDP-43 Fusion Protein Ag1231
Conjugate	Unconjugated
Form	Liquid
Concentration	0.31 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 45 kDa protein as well as all post translationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. Recently, TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design.

Target Information

TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.