Proteintech

TDP-43 Polyclonal Antibody

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TDP-43 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 18280-1-AP (TDP-43 (full length) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

TDP-43 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 18280-1-AP (TDP-43 (full length) antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

TDP-43 Antibody in Western Blot (WB) — K-562 cells were subjected to SDS PAGE followed by western blot with 18280-1-AP (TDP-43 (full length) antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

TDP-43 Antibody in Flow Cytometry (Flow) — 1X10^6 SH-SY5Y cells were stained with 0.2ug TDP-43 (full length) antibody (18280-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

TDP-43 Antibody in Immunoprecipitation (IP) — IP result of anti-TARDBP (IP:18280-1-AP, 3ug; Detection:18280-1-AP 1:1500) with K-562 cells lysate 6000ug.

Product Details

18280-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:500	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 1 publication 1 publication
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Full length TDP-43 Fusion Protein Ag13118 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues.

Target Information

TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates.

Nature neuroscience

Laferrière F,Maniecka Z,Pérez-Berlanga M,Hruska-Plochan M,Gilhespy L,Hock EM,Wagner U,Afroz T,Boersema PJ,Barmettler G,Foti SC,Asi YT,Isaacs AM,Al-Amoudi A,Lewis A,Stahlberg H,Ravits J,De Giorgi F,Ichas F,Bezard E,Picotti P,Lashley T,Polymenidou M

Tue Jan 01 00:00:00 EST 2019

Viral delivery of C9orf72 hexanucleotide repeat expansions in mice leads to repeat-length-dependent neuropathology and behavioural deficits.

Disease models & mechanisms

Herranz-Martin S,Chandran J,Lewis K,Mulcahy P,Higginbottom A,Walker C,Valenzuela IMY,Jones RA,Coldicott I,Iannitti T,Akaaboune M,El-Khamisy SF,Gillingwater TH,Shaw PJ,Azzouz M