Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
TDP-43 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 10
TDP-43 Antibody (PA1-16996) in ICC/IF
Immunocytochemistry analysis of TDP-43 in HeLa cells. Samples were incubated with TDP-43 polyclonal antibody (Product # PA1-16996) using a dilution of 2 µg/mL overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Product Details
PA1-16996
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Non-human primate,
Xenopus,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide to a C-terminal region [within residues 350-414] of the human TARDBP protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: Hela whole cell extract, antigen standard for TARDBP (transient overexpression lysate).
Target Information
TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: OTTHUMP00000002171; OTTHUMP00000002173; RP4-635E18.2; TAR DNA-binding protein 43; TAR DNA-binding protein-43; TDP-43
Gene Aliases: 1190002A23Rik; ALS10; C85084; RCJMB04_5g9; TARDBP; TDP-43; TDP43; wu:fb77f02; wu:fc52g10; XELAEV_18035188mg
UniProt ID: (Chicken) Q5ZLN5, (Human) Q13148, (Mouse) Q921F2
Entrez Gene ID: (Chicken) 419453, (Human) 23435, (Mouse) 230908, (Zebrafish) 325052, (Xenopus) 380329
DNA binding
RNA binding
nucleotide binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
mRNA 3'-UTR binding
protein binding
identical protein binding
poly(A) RNA binding
nucleic acid binding
negative regulation of protein phosphorylation
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
mRNA processing
RNA splicing
negative regulation of gene expression
nuclear fragmentation involved in apoptotic nuclear change
negative regulation by host of viral transcription
positive regulation of transcription from RNA polymerase II promoter
regulation of cell cycle
3'-UTR-mediated mRNA stabilization
nuclear inner membrane organization
regulation of protein stability
regulation of circadian rhythm
rhythmic process
positive regulation of insulin secretion
response to endoplasmic reticulum stress
axonogenesis
skeletal muscle tissue development
neuromuscular junction development
locomotory behavior
blood vessel morphogenesis
axon extension
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support