Invitrogen

TEAD1 Polyclonal Antibody

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TEAD1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of TEAD1 in COLO205 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with TEAD1 polyclonal antibody (Product # PA5-106477) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

TEAD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded TEAD1 in mouse pancreas tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with TEAD1 polyclonal antibody (Product # PA5-106477) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

TEAD1 Antibody in Western Blot (WB) — Western blot analysis of TEAD1 in COLO205 cell lysate (left lane: treated with blocking peptide). Samples were incubated with TEAD1 polyclonal antibody (Product # PA5-106477).

Product Details

PA5-106477

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human TEAD1(Accession P28347), corresponding to amino acid residues R85-T113. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2854146

Target Information

TEF1 (Transcriptional enhancer factor 1), a member of the TEA/ATTS domain family, is a nuclear protein that is expressed in numerous cell types and plays a role in controlling the expression of numerous genes. TEF family members have a highly conserved DNA-binding domain TEF-1 binds to GT-IIC, SphI/II and M-CAT. TEF-1 also binds to the proximal regulatory element (PRE) of transforming growth factor-alpha, a member of the EGF family that is overexpressed in many types of cancer. Furthermore, TEF-1 represses transcription in placental cells. In vitro, TEF-1 is phosphorylated by several PKC isozymes. TEF-1 is phosphorylated in vivo at serine and threonine residues. Phosphorylation of TEF-1, both in vivo and in vitro, results in a reduction in its DNA-binding capability, which suggests a potential role for TEF-1 in PKC inhibition. TEF-1 also complexes with larger tumor antigen (TAg), and may thus have a role in tumorigenesis. Dimerization of TEF-1 may be important for TEF-1 to function as a regulator of gene transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: N TEAD1; NTEF-1; NTEF1; Protein GT-IIC; TCF-13; TEA domain family member 1; TEA domain family member 1 (SV40 transcriptional enhancer factor); TEAD-1; Transcription factor 13; transcriptional enhancer factor 1; Transcriptional enhancer factor TEF-1

Gene Aliases: 2610024B07Rik; AA; B230114H05Rik; Gtrgeo5; mTEF-1; NTEF-1; REF1; TCF-13; TCF13; TEAD-1; TEAD1; TEF-1; TEF1

UniProt ID: (Human) P28347

Entrez Gene ID: (Human) 7003, (Mouse) 21676, (Rat) 361630

Molecular Function: DNA-binding transcription factor