Invitrogen

TEK Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View Details

View all (27) TIE2 (TEK) antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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TEK Antibody in Western Blot (WB) — Western blot was performed using Anti-TEK Polyclonal Antibody (Product # PA5-23082) and a 150 kDa band corresponding to TEK was observed across cell lines and tissues tested except U-87 MG and Mouse Kidney which are reported to be negative. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), MCF-7 (Lane 2), U-87 MG (Lane 3), HUVEC (Lane 4), HUVEC treated with Cobalt Chloride (75umol/ml for 24hr) (Lane 5), HUVEC treated with IL-1beta (1ng/ml, 24hr) (Lane 6), Mouse lung (Lane 7) and Mouse kidney (Lane 8) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (PA5-23082,1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

TEK Antibody in Cell treatment — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using TEK Polyclonal Antibody (Product # PA5-23082), shows increased expression of proteins in HUVEC cell upon treatment with Cobalt Chloride and IL-1 beta. {TM}

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Western Blot (WB)

1:1000 - 1:2500

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide made to an internal region of the human TIE2 protein (within residues 250-400).

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11153466

Product Specific Information

Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Target Information

TIE2, also known as TEK tyrosine kinase, TIE2 gene is mapped to 9p21.2. This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Angiopoietin-1 receptor; CD202b; CD202b antigen; EC 2.7.10; EC 2.7.10.1; Endothelial tyrosine kinase; hTIE2; HYK; mTIE2; p140 TEK; STK1; TEK tyrosine kinase, endothelial; TEKT1; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2

Gene Aliases: AA517024; CD202B; Hyk; STK1; TEK; TIE-2; TIE2; VMCM; VMCM1

UniProt ID: (Human) Q02763, (Mouse) Q02858

Entrez Gene ID: (Human) 7010, (Mouse) 21687

Function(s)