Invitrogen

TEM5 Polyclonal Antibody

TEM5 Antibody in Immunohistochemistry (IHC)

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TEM5 Antibody (PA5-20442) in IHC

Immunohistochemistry of TEM5 in human bladder tissue with TEM5 Polyclonal Antibody (Product # PA5-20442) at 5 µg/mL.

TEM5 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

TEM5 Antibody — Figure 4 GPR124-dependent signaling controls pericyte migration. ( A ) Diagram of the knockdown system. ( B ) Immunoblot analysis of GPR124 protein level in control HBVPs and in HBVPs transduced with a lentivirus expressing an shRNA to silence endogenous GPR124. ( C ) Quantitative analyses of the results as shown in ( B ) and presented in the bar graph as the densitometry ratio of the control from four independent experiments. ( D ) Diagram of the overexpression system. ( E ) Immunoblot analysis of GPR124 proteins in HBVPs transfected with either control or gene construct encoding GPR124 (FLAG-GPR124, MOI = 50 and 100) to confirm overexpression of GPR124. ( F ) Quantitative analyses of the results as shown in ( E ) and presented in the bar graph as the densitometry ratio of the control from four independent experiments. ( G ) Cell viability was measured by the CCK8 assay in Ctrl-HBVPs and shGPR124-HBVP (n = 4). (H) Representative images of control HBVPs (control), shGPR124 HBVPs (shGPR124) and GPR124-overexpressing HBVPs (GPR124 OE) at the beginning (t = 0 h) and end (t = 24 h) of the wound healing assay. The dashed lines (black) indicate the edges of the wound immediately after scratch. The dashed lines (red) outline the area of cell migration. (I) Quantification of relative migration in a wound healing assay after 24 h (normalized to control values; n = 4 in each group). (J) Schematic representation of the microfluidic migration chamber used for observing the chemotactic re {KD}

Product Details

PA5-20442

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

20 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 14 amino acid synthetic peptide near the center of human TEM5. The immunogen is located within amino acids 560-610 of TEM5.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11152277

Product Specific Information

A suggested positive control is rat kidney tissue lysate.

PA5-20442 can be used with blocking peptide PEP-0559.

Predicted species reactivity based on immunogen sequence: Mouse: (85%).

Target Information

Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. These include TEMs such as TEM1, TEM5, TEM7 and TEM8. TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GPR124 facilitates pericyte polarization and migration by regulating the formation of filopodia during ischemic injury.

Theranostics

Chen DY,Sun NH,Lu YP,Hong LJ,Cui TT,Wang CK,Chen XH,Wang SS,Feng LL,Shi WX,Fukunaga K,Chen Z,Lu YM,Han F

PA5-20442 was used in Western Blotting to provide the first evidence for a role of GPR124 in pericyte migration under ischemic conditions and suggest that GPR124 was essential for Cdc42 activation and filopodia formation.

Mon Aug 31 00:00:00 UTC 2020

GPR124 facilitates pericyte polarization and migration by regulating the formation of filopodia during ischemic injury.

Theranostics

Chen DY,Sun NH,Lu YP,Hong LJ,Cui TT,Wang CK,Chen XH,Wang SS,Feng LL,Shi WX,Fukunaga K,Chen Z,Lu YM,Han F

Mon Aug 31 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: Adhesion G protein-coupled receptor A2; DKFZp434C211; DKFZp434J0911; FLJ14390; G protein-coupled receptor 124; G-protein coupled receptor 124; Tumor endothelial marker 5

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Gene Aliases: ADGRA2; GPR124; KIAA1531; TEM5

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UniProt ID: (Human) Q9UFY4

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Entrez Gene ID: (Human) 25960, (Rat) 100363275

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Function(s)

G-protein coupled receptor activity protein binding G-protein coupled receptor

Process(es)

sprouting angiogenesis cell surface receptor signaling pathway G-protein coupled receptor signaling pathway central nervous system development positive regulation of endothelial cell migration endothelial cell migration regulation of angiogenesis regulation of chemotaxis regulation of establishment of blood-brain barrier negative regulation of vascular endothelial growth factor signaling pathway angiogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TEM5 Polyclonal Antibody

TEM5 Antibody (PA5-20442) in IHC

Product Details

PA5-20442

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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