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TET3 Antibody (PA5-120543) in WB
Western blot analysis of TET3 in extracts of mESCs cells. Samples were incubated with TET3 Polyclonal antibody (Product # PA5-120543) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-120543
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1551-1650 of human TET3 (NP_0012744201)
Conjugate
Form
Liquid
Concentration
3.43 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914115
Product Specific Information
Positive test controls include: mESCs. The target is usually found in the following locations: Cytoplasm, Nucleus.
Immunogen sequence: GFQDKLWNPM KGEEGRIPAA GASQLDRAWQ SFGLPLGSSE KLFGALKSEE KLWDPFSLEE GPAEEPPSKG AVKEEKGGGG AEEEEEELWS DSEHNFLDEN
Target Information
TET3, a member of the ten-eleven-translocation (TET) family of genes, is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine and is most abundantly expressed in hematopoietic cells. Unlike the related TET2 protein, mutations in TET3 have not been observed in any myeloid malignancies. TET3 has been shown to be involved in the demethylation of zygotic DNA before the first mitosis and has been suggested to be involved in the epigenetic reprogramming of the zygotic paternal DNA following natural fertilization and may also contribute to somatic cell nuclear reprogramming during animal cloning.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Methylcytosine dioxygenase TET3; MGC22014; probable methylcytosine dioxygenase TET3; putative methylcytosine dioxygenase; Tet methylcytosine deoxygenase 3 isoform; tet oncogene family member 3
Gene Aliases: B430006D22Rik; BC037432; D230004J03Rik; hCG_40738; KIAA0401; TET3
UniProt ID: (Human) O43151, (Mouse) Q8BG87
Entrez Gene ID: (Human) 200424, (Mouse) 194388
Performance Guarantee
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