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Invitrogen
TFF1 Polyclonal Antibody
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FIGURE: 1 / 6
TFF1 Antibody (PA1-28875) in ICC/IF
Immunofluorescence analysis of Trefoil factor 1 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TFF1 Polyclonal Antibody (Product # PA1-28875) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Prod... View More
Product Details
PA1-28875
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide representing a highly antigenic site near the C-terminus of the pS2 protein.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: About 70% of breast carcinomas show partial staining of tumor cells..
Target Information
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence; estrogen receptor; gastrointestinal trefoil protein pS2; hP1.A; PNR-2; Polypeptide P1.A; Protein pS2; Trefoil factor 1; unnamed protein product
Gene Aliases: BCEI; D21S21; HP1.A; HPS2; pNR-2; PS2; TFF1
UniProt ID: (Human) P04155
Entrez Gene ID: (Human) 7031
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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