Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
TFIP11 Antibody (PA5-97175) in WB
Western blot analysis of TFIP11 in extracts of various cell lines. Samples were incubated with TFIP11 Polyclonal antibody (Product # PA5-97175) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-97175
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human TFIP11 (NP_0362751)
Conjugate
Form
Liquid
Concentration
2.31 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808977
Product Specific Information
Immunogen sequence: MSLSHLYRDG EGRIDDDDDE RENFEITDWD LQNEFNPNRQ RHWQTKEEAT YGVWAERDSD DERPSFGGKR ARDYSAPVNF ISAGLKKGAA EEAELEDSDD EEKPVKQDDF PKDFGPRKLK TGGNFKPSQK GFAGGTKSFM; Positive Samples: LO2, HeLa, A-431, MCF-7, HT-1080, Mouse brain, Mouse liver; Cellular Location: Cytoplasm, Nucleus
Target Information
Involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. Intron turnover seems to proceed through reactions in two lariat-intron associated complexes termed Intron Large (IL) and Intron Small (IS). In cooperation with DHX15 seems to mediate the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns. May play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Septin and tuftelin-interacting protein 1; STIP-1; Tuftelin-interacting protein 11; tuftelin-interacting protein 33; Tuftelin-interacting protein 39; tuftelin-interacting protein, 39 kD
Gene Aliases: 2810002G02Rik; AF097181; AW046167; bK445C9.6; hNtr1; HSPC006; NTR1; Spp382; Srr1; STIP; TFIP11; TIP33; TIP39
UniProt ID: (Human) Q9UBB9, (Mouse) Q9ERA6
Entrez Gene ID: (Human) 24144, (Mouse) 54723
spliceosomal complex disassembly
mRNA splicing, via spliceosome
regulation of transcription, DNA-templated
RNA processing
biomineral tissue development
negative regulation of protein complex assembly
protection from non-homologous end joining at telomere
negative regulation of protein binding
negative regulation of DNA ligase activity
negative regulation of double-strand break repair via nonhomologous end joining
mRNA processing
RNA splicing
cell differentiation
extracellular matrix organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support