Western blot analysis of HeLa Whole Cell Lysate using PA5-19809, TFPI primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19809 staining of Jurkat Whole Cell Lysate 10 ug. at a dilution of 1ug/ml (Lane 2). PA5-19809 staining of A431 Whole Cell Lysate at a dilution of 1ug/ml (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TFPI.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with rat, cow, chimpanzee, rhesus monkey and orangutan based on sequence homology.
This gene encodes a protease inhibitor that regulates the tissue factor and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.