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Invitrogen
TFPI2 Polyclonal Antibody
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FIGURE: 1 / 4
TFPI2 Antibody (PA5-95444) in Flow
Flow cytometry analysis of TFPI2 in A549 cells using TFPI2 Polyclonal Antibody (Product # PA5-95444), shown in overlay histogram (blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum, and incubated with the primary antibody (1 μg/1x10^6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibo... View More
Product Details
PA5-95444
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TFPI2 (70-105aa EGNANNFYTWEACDDACWRIEKVPKVCRLQVSVDDQ).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807247
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: mouse spleen tissue, HELA whole cell, human placenta tissue, MCF-7 whole cell. IHC: human placenta tissue. Flow: U87 cell, A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
TFPI2 may play a role in the regulation of plasmin-mediated matrix remodeling. It inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. TFPI2 has no effect on thrombin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Placental protein 5; PP5; PP5FLJ21164; REF-1; retinal pigment epithelium cell factor 1; TFPI-2; TFPI-2REF1; Tissue factor pathway inhibitor 2
Gene Aliases: AV000670; PP5; PP5/TFPI-2; REF1; TFPI-2; TFPI2
UniProt ID: (Human) P48307, (Mouse) O35536
Entrez Gene ID: (Human) 7980, (Mouse) 21789
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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