|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human TGF-alpha|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-2 ug/ml|
|Functional Assay (FN)||2.5-3.7 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5 ug/ml|
|Western Blot (WB)||0.1-0.2ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. This antibody may be used in Western Blotting applications under either reducing or non-reducing conditions.
Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% sodium azide is recommended (unless the product will be used for functional studies). Prior to reconstitution, store at 4°C. After reconstitution, store undiluted at -20°C, avoiding freeze/thaw cycles.
Transforming growth factors (TGFs) are biologically active polypeptides that reversibly confer the transformed phenotype on cultured cells. TGF-alpha shows about 40% sequence homology with epidermal growth factor (EGF; MIM 131530) and competes with EGF for binding to the EGF receptor ., stimulating its phosphorylation and producing a mitogenic response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.