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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TGF beta-1 Antibodies

          Invitrogen

          TGF beta-1 Polyclonal Antibody

          2 References
          View all (67) TGF beta-1 antibodies

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          Cite TGF beta-1 Polyclonal Antibody

          TGF beta-1 Polyclonal Antibody

          Product Details

          PA1-9574

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:5,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          1:2,000-1:5,000
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Pig, Rat

          Published species

          Rat

          Host/Isotype

          Chicken / IgY

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Peptide based on mouse and human TGF beta-1 protein.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2201915

          Product Specific Information

          PA1-9574 detects TGFb1 in human, mouse and rat samples.

          PA1-9574 has been successfully used in Western blot, Immunohistochemistry, Immunocytochemistry, and ELISA procedures. By Western blot, PA1-9574 detects a 25 kDa protein representing TGFb1.

          PA1-9574 immunogen is a peptide based on mouse and human TGFb1 protein (RKPKVEQLSNMIVRS).

          Store 4c for 1 week and -20c for long term storage.

          Target Information

          TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: cytokine; latency-associated peptide; prepro-transforming growth factor beta-1; regulatory protein; TGF b; TGF b 1; TGF beta; tgf beta 1; TGF beta-1; TGF beta-1 protein; tgf beta1; TGF β; TGF β 1; TGF β1; TGF-B1; TGF-beta 1; TGF-beta 1 protein; TGF-beta-1; TGFβ1; Transforming growth factor; transforming growth factor b1; transforming growth factor beta; Transforming growth factor beta-1; Transforming growth factor beta-1 proprotein; transforming growth factor β1; transforming growth factor, beta 1 (Camurati-Engelmann disease); transforming growth factor, beta-1; transforming growth factor-beta 1; unnamed protein product

          View more View less

          Gene Aliases: CED; DPD1; LAP; TGF-BETA-1; TGF-beta1; TGFB; Tgfb-1; TGFB1; TGFbeta; TGFbeta1

          View more View less

          UniProt ID: (Human) P01137, (Pig) P07200, (Rat) P17246, (Mouse) P04202

          View more View less

          Entrez Gene ID: (Human) 7040, (Pig) 397078, (Rat) 59086, (Mouse) 21803

          View more View less

          Function(s)
          glycoprotein binding antigen binding type II transforming growth factor beta receptor binding cytokine activity transforming growth factor beta receptor binding protein binding growth factor activity enzyme binding type I transforming growth factor beta receptor binding type III transforming growth factor beta receptor binding protein homodimerization activity protein serine/threonine kinase activator activity protein heterodimerization activity protein N-terminus binding identical protein binding growth factor
          Process(es)
          protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter MAPK cascade ureteric bud development response to hypoxia epithelial to mesenchymal transition negative regulation of protein phosphorylation positive regulation of protein phosphorylation regulation of sodium ion transport chondrocyte differentiation hematopoietic progenitor cell differentiation connective tissue replacement involved in inflammatory response wound healing adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains tolerance induction to self antigen platelet degranulation protein phosphorylation protein export from nucleus ATP biosynthetic process phosphate-containing compound metabolic process cellular calcium ion homeostasis inflammatory response cell cycle arrest mitotic cell cycle checkpoint epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly SMAD protein import into nucleus Notch signaling pathway negative regulation of neuroblast proliferation salivary gland morphogenesis endoderm development female pregnancy aging negative regulation of DNA replication positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration response to radiation response to wounding response to glucose defense response to fungus, incompatible interaction positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression negative regulation of extracellular matrix disassembly positive regulation of epithelial to mesenchymal transition macrophage derived foam cell differentiation positive regulation of fibroblast migration positive regulation of peptidyl-threonine phosphorylation positive regulation of pathway-restricted SMAD protein phosphorylation negative regulation of macrophage cytokine production oligodendrocyte development cell growth regulation of striated muscle tissue development cell migration regulation of transforming growth factor beta receptor signaling pathway evasion or tolerance of host defenses by virus negative regulation of cell-cell adhesion hyaluronan catabolic process negative regulation of ossification negative regulation of cell growth regulation of cell migration positive regulation of cell migration positive regulation of bone mineralization negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of histone deacetylation organ regeneration positive regulation of protein complex assembly positive regulation of exit from mitosis lipopolysaccharide-mediated signaling pathway positive regulation of cellular protein metabolic process response to estradiol response to progesterone regulation of interleukin-23 production negative regulation of interleukin-17 production positive regulation of interleukin-17 production receptor catabolic process positive regulation of superoxide anion generation mononuclear cell proliferation positive regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation response to vitamin D response to laminar fluid shear stress positive regulation of histone acetylation positive regulation of protein dephosphorylation negative regulation of T cell proliferation regulation of protein import into nucleus positive regulation of protein import into nucleus positive regulation of odontogenesis response to drug myelination regulation of apoptotic process myeloid dendritic cell differentiation T cell homeostasis positive regulation of apoptotic process positive regulation of vascular permeability positive regulation of MAP kinase activity protein kinase B signaling positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration positive regulation of phosphatidylinositol 3-kinase activity ossification involved in bone remodeling regulatory T cell differentiation cell-cell junction organization positive regulation of regulatory T cell differentiation negative regulation of cell differentiation negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter active induction of host immune response by virus positive regulation of fibroblast proliferation positive regulation of isotype switching to IgA isotypes lymph node development digestive tract development negative regulation of skeletal muscle tissue development inner ear development positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation positive regulation of protein secretion positive regulation of peptidyl-tyrosine phosphorylation negative regulation of phagocytosis positive regulation of chemotaxis positive regulation of NF-kappaB transcription factor activity regulation of binding regulation of DNA binding positive regulation of smooth muscle cell differentiation negative regulation of release of sequestered calcium ion into cytosol positive regulation of cell division positive regulation of protein kinase B signaling regulation of blood vessel remodeling face morphogenesis frontal suture morphogenesis pathway-restricted SMAD protein phosphorylation regulation of SMAD protein import into nucleus positive regulation of SMAD protein import into nucleus SMAD protein signal transduction mammary gland branching involved in thelarche branch elongation involved in mammary gland duct branching regulation of branching involved in mammary gland duct morphogenesis negative regulation of gene silencing by miRNA regulation of cartilage development lens fiber cell differentiation response to cholesterol positive regulation of cell cycle arrest cellular response to organic cyclic compound cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus extracellular matrix assembly positive regulation of branching involved in ureteric bud morphogenesis extrinsic apoptotic signaling pathway negative regulation of hyaluronan biosynthetic process positive regulation of extracellular matrix assembly positive regulation of NAD+ ADP-ribosyltransferase activity positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of protein localization to plasma membrane negative regulation of production of miRNAs involved in gene silencing by miRNA positive regulation of receptor clustering regulation of epithelial to mesenchymal transition involved in endocardial cushion formation regulation of actin cytoskeleton reorganization positive regulation of transcription regulatory region DNA binding positive regulation of cardiac muscle cell differentiation vasculogenesis neural tube closure aortic valve morphogenesis positive regulation of microglia differentiation osteoclast differentiation membrane protein intracellular domain proteolysis negative regulation of cytolysis muscle cell cellular homeostasis ventricular cardiac muscle tissue morphogenesis negative regulation of biomineral tissue development positive regulation of ERK1 and ERK2 cascade positive regulation of canonical Wnt signaling pathway positive regulation of production of miRNAs involved in gene silencing by miRNA transforming growth factor beta receptor signaling pathway involved in heart development embryonic liver development regulation of cell growth morphogenesis of a branching structure cell activation cell proliferation response to organic substance response to organic cyclic compound T cell differentiation lung development mammary gland development wound healing T cell activation regulation of cell proliferation regulation of MAPK cascade negative regulation of immune response negative regulation of T cell activation cellular response to growth factor stimulus positive regulation of protein localization to nucleus regulation of miRNA metabolic process skeletal system development CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment defense response skeletal muscle tissue development organ morphogenesis regulation of gene expression regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation growth cell development T-helper 17 cell lineage commitment
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