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FIGURE: 1 / 1
TGIF Antibody (PA5-120515) in WB
Western blot analysis of TGIF in extracts of various cell lines. Samples were incubated with TGIF Polyclonal antibody (Product # PA5-120515) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-120515
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 250-340 of human TGIF1 (NP_733796.2).
Conjugate
Form
Liquid
Concentration
1.14 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914087
Product Specific Information
Positive test controls include: HepG2, MCF7, K-562, U-251MG, Mouse liver, Mouse testis, Rat uterus.
Immunogen sequence: SSVESVMGIK NFMPALEETP FHSCTAGPNP TLGRPLSPKP SSPGSVLARP SVICHTTVTA LKDVPFSLCQ SVGVGQNTDI QQIAAKNFTD T
Target Information
TGIF is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5'-TG-3'-interacting factor 1; Homeobox protein TGIF1; TALE family homeobox; TALE homeobox TG-interacting factor; TG interacting factor 1; TGFB-induced factor homeobox 1; transforming growth factor-beta-induced factor
Gene Aliases: AA959811; AI462167; HPE4; Tfig; TGIF; TGIF1
UniProt ID: (Human) Q15583, (Mouse) P70284
Entrez Gene ID: (Human) 7050, (Mouse) 21815, (Rat) 316742
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
co-SMAD binding
DNA binding
chromatin binding
JUN kinase binding
homeodomain transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
multicellular organism development
response to drug
cellular response to growth factor stimulus
neural tube closure
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
determination of left/right symmetry
negative regulation of cell proliferation
dorsal/ventral pattern formation
regulation of gastrulation
negative regulation of gene expression
nodal signaling pathway
positive regulation of neuron differentiation
positive regulation of fibroblast proliferation
negative regulation of retinoic acid receptor signaling pathway
retina development in camera-type eye
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