Invitrogen

TGM2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

1 Published Figure

1 Reference

View all (32) TGM2 antibodies

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Datasheet

Questions & Answers

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TGM2 Antibody in Western Blot (WB) — Western blot was performed using Anti-TGM2 Polyclonal Antibody(Product # PA5-23219) and a 78kDa band corresponding to TGM2 was observed across cell lines and tissue tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), MDA-MB-231 (Lane 3), MCF7 (Lane 4), LNCaP (Lane 5) and DU 145 (Lane 6).Tissue extracts of Mouse Testis (Lane 7) and Mouse Placenta (Lane 8), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

TGM2 Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of TGM2 was observed in High in MDA-MB-231 and low or negative in MCF-7. Similarly expression of TGM2 was High in DU 145 and low or negative in LnCaP. In tissues expression was high in Mouse Placenta and Low or negative in Mouse Testis using Anti-TGM2 Polyclonal Antibody (Product # PA5-23219) in Western Blot.(Ref :https://doi.org/10.1016/j.ejca.2014.02.014, DOI:10.1093/carcin/bgm280). {RE}

TGM2 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with TGM2 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-TGM2 Polyclonal Antibody (Product # PA5-23219). {KD}

TGM2 Antibody — Fig. 3 Intracellular polymerization and in situ aggregation of peptide monomers. a TG2-catalyzed intracellular polymerization of peptide monomers was monitored by fluorescent resonance energy transfer (FRET) technique. The 3D confocal images displayed the FRET effect of control, P4 , P7 , and P9 inside HeLa cells at 12 h-post treatment. The feed ratio of FITC-peptide (fluorescein isothiocyannate-peptide) and CO-peptide (coumarin-peptide) was 1:1. b Confocal images displayed the nanostructures (green) formed from P4 (at 4 degC) and ( P7 , P9 ) (at 37 degC). P8 , P4 , P7 , and P9 were ELP random coil, UCST-type ELP, LCST-type ELP, and ELP gel, respectively. Cell membrane, red. Scale bar, 30 mum. c The ultrathin cell sections of HeLa cells. Fe-chelated P 18 was used to identify the formed nanostructures and for contrast enhancement. Red arrows indicated P 18 -P4 3D nanoparticles; red dotted circle indicated P 18 -P9 gel. Scale bar, 1 mum. d Energy dispersive spectrometry (EDS) of the nanostructures formed from P 18 -P4 . The Fe element signal (red highlighted) made the P 18 -P4 from the biological background stand out. e MCF-7 and HeLa cells were lysed. TG2 protein was examined by western blotting. f Fluorescein isothiocyannate (FITC)-labeled P7 and P9 were incubated with MCF-7 or HeLa cells for fluorescence imaging. FITC-P7 and FITC-P9 , green; nucleus, blue. Scale bar, 30 mum. g The expression of TG2 protein in SH-SY5Y cells was modulated by normoxia (N) and hypoxia (H) condit

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

Western Blot (WB)

2-5 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide (TVEGAGLTEEQKTVEIPDPVEAGc (aa623- 645).) of Transglutaminase 2 (TG2) protein.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2540744

Target Information

Transglutaminase (TGase) catalyses the crosslink of proteins by forming e-(g-glutamyl) lysine isopeptide bonds and requires the binding of Ca2+ for its activity. In mammals, eight distinct TGase isoenzymes have been identified. Tissue transglutaminase (tTGase), also known as TGase 2, has four distinct domains: N-terminal b-sandwich, catalytic core and two C-terminal b-barrel domains. tTGase may have a role in cell death, cell proliferation, cell differentiation, and receptor-mediated endocytosis. In the Alzheimer's disease brain, the elevated tTGase activity is manifested by polymerization of a number of proteins, including Ab peptide, b-amyloid precursor protein and the tau protein, with formation of neurofibrillary tangles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C polypeptide, protein-glutamine-gamma-glutamyltransferase; G alpha h; G-ALPHA-h; GNAH; Protein-glutamine gamma-glutamyltransferase 2; RP5-1054A22.2; TG(C); TGase C; TGase H; TGase-2; TGase-H; Tissue transglutaminase; Transglutaminase C; Transglutaminase H; Transglutaminase-2

Gene Aliases: TG(C); TGC; TGM2

UniProt ID: (Human) P21980

Entrez Gene ID: (Human) 7052

Molecular Function: acyltransferase transferase