Invitrogen

TGM2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (47) TGM2 antibodies

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FIGURE: 1 / 13

TGM2 Antibody (PA5-23219) in WB

Western blot was performed using Anti-TGM2 Polyclonal Antibody(Product # PA5-23219) and a 78kDa band corresponding to TGM2 was observed across cell lines and tissue tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), MDA-MB-231 (Lane 3), MCF7 (Lane 4), LNCaP (Lane 5) and DU 145 (Lane 6).Tissue extracts of Mouse Testis (Lane 7) and Mouse Placenta (Lane 8), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Produc... View More

Product Image Gallery

TGM2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of TGM2 was performed using 70% confluent log phase MDA-MB-231 and MCF7 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with TGM2 Polyclonal Antibody (Product # PA5-23219) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Alexa Fluor Plus 488 donkey anti-rabbit IgG secondary antibody -A32790), (1:2,000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoplasmic localization. Panel e represents MCF7 cells having no expression of TGM2. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

TGM2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of TGM2 was observed in High in MDA-MB-231 and low or negative in MCF-7. Similarly expression of TGM2 was High in DU 145 and low or negative in LnCaP. In tissues expression was high in Mouse Placenta and Low or negative in Mouse Testis using Anti-TGM2 Polyclonal Antibody (Product # PA5-23219) in Western Blot.(Ref :https://doi.org/10.1016/j.ejca.2014.02.014, DOI:10.1093/carcin/bgm280). {RE}

Product Details

PA5-23219

Applications

Tested Dilution

Publications

Western Blot (WB)

2-5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

Immunocytochemistry (ICC/IF)

5 µg/mL

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A portion of amino acids 600-650 of human Transglutaminase 2

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2540744

Target Information

Transglutaminase (TGase) catalyses the crosslink of proteins by forming e-(g-glutamyl) lysine isopeptide bonds and requires the binding of Ca2+ for its activity. In mammals, eight distinct TGase isoenzymes have been identified. Tissue transglutaminase (tTGase), also known as TGase 2, has four distinct domains: N-terminal b-sandwich, catalytic core and two C-terminal b-barrel domains. tTGase may have a role in cell death, cell proliferation, cell differentiation, and receptor-mediated endocytosis. In the Alzheimer's disease brain, the elevated tTGase activity is manifested by polymerization of a number of proteins, including Ab peptide, b-amyloid precursor protein and the tau protein, with formation of neurofibrillary tangles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Intracellular construction of topology-controlled polypeptide nanostructures with diverse biological functions.

Nature communications

Li LL,Qiao SL,Liu WJ,Ma Y,Wan D,Pan J,Wang H

Published figure using TGM2 polyclonal antibody (Product # PA5-23219) in Western Blot

Thu Nov 02 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: C polypeptide, protein-glutamine-gamma-glutamyltransferase; Erythrocyte transglutaminase; G alpha h; G(h); G-ALPHA-h; GNAH; Heart G alpha(h); hhG alpha(h); Isopeptidase TGM2; Protein G alpha(h); Protein-glutamine deamidase TGM2; Protein-glutamine dopaminyltransferase TGM2; Protein-glutamine gamma-glutamyltransferase 2; Protein-glutamine histaminyltransferase TGM2; Protein-glutamine noradrenalinyltransferase TGM2; Protein-glutamine serotonyltransferase TGM2; RP5-1054A22.2; TG(C); TG2; TGase C; TGase H; TGase II; TGase-2; TGase-H; Tissue transglutaminase; Tissue type transglutaminase; Transglutaminase 2 C polypeptide; Transglutaminase C; Transglutaminase H; Transglutaminase II; Transglutaminase-2; tTG

Gene Aliases: TG(C); TGC; TGM2

UniProt ID: (Human) P21980

Entrez Gene ID: (Human) 7052

Molecular Function: transferase

Function(s)

phospholipase C-activating G-protein coupled receptor signaling pathway peptide cross-linking negative regulation of endoplasmic reticulum calcium ion concentration positive regulation of apoptotic process apoptotic cell clearance positive regulation of cell adhesion positive regulation of mitochondrial calcium ion concentration branching involved in salivary gland morphogenesis salivary gland cavitation

Process(es)

protein-glutamine gamma-glutamyltransferase activity protein binding metal ion binding