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Invitrogen
TGR5 Polyclonal Antibody
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FIGURE: 1 / 6
TGR5 Antibody (PA5-23182) in ICC/IF
Immunocytochemistry analysis of TGR5 in HEK293 cells. Samples were incubated with TGR5 polyclonal antibody (Product # PA5-23182) using a dilution of 2 µg/mL overnight at 4°C followed by anti-rabbit DyLight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. Alpha tubulin (DM1A) was used as a co-stain at a 1:1,000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue)... View More
Product Details
PA5-23182
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide from C-terminus of the human TGR5/GPBAR1 protein.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Sequence homology: Bovine (86%).
Target Information
This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BG37; G protein-coupled receptor; G protein-coupled receptor TGR5; G-protein coupled bile acid receptor 1; G-protein coupled bile acid receptor BG37; G-protein coupled receptor GPCR19; GPCR HBG37; hBG37; hGPCR19; M-BAR; membrane bile acid receptor; Membrane-type receptor for bile acids; unnamed protein product
Gene Aliases: BG37; GPBAR1; GPCR; GPCR19; GPR131; M-BAR; TGR5
UniProt ID: (Human) Q8TDU6, (Rat) Q80T02, (Mouse) Q80SS6
Entrez Gene ID: (Human) 151306, (Rat) 338443, (Mouse) 227289
signal transduction
G-protein coupled receptor signaling pathway
cell surface bile acid receptor signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to bile acid
positive regulation of cholangiocyte proliferation
regulation of bicellular tight junction assembly
bile acid signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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