Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human liver stained using a THEM2 polyclonal antibody (Product # PA5-23272) at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Human, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (123 KATGKLIAQGRHTKHLGN 140) of THEM2 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||0.05-0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
THEM2 is a 140 amino acid enzyme with hydrolase activity belonging to the thioesterase superfamily and UPF0152 (paal) family. Human THEM2 contains a hotdog-fold and forms a back-to-back tetramer with the active site located in an inter-subunit cleft. The biological function of THEM2 is yet unknown. The role of THEM2 and Pax3 (paired box gene 3) as PC-TP-interacting proteins has been determined by using yeast two-hybrid screening. Small interference RNA silencing data suggest that the gene encoding THEM2 is essential for the cell sustained proliferation. THEM2 is highly expressed in kidney, and moderately in liver, brain, large intestine, and small intestine. THEM2 is co-localized with microtubules and is implicated in the genetic background of developmental dyslexia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.