PA1-211A detects thyroid receptor (TR) from human, mouse, rat and chicken samples. This antibody does not detect TRv alpha -2 or TR beta-1.
PA1-211A has been successfully used in Western blot, IHC-P, ICC/IF and gel shift procedures. By Western blot, this antibody detects a ~48 kDa protein representing recombinant human TR alpha-1 expressed in E. coli. Gel shift experiments show that this product reacts with TR alpha-1 homodimers and TR alpha-1/retinoic X receptor (RXR) heterodimers.
PA1-211A immunizing peptide corresponds to amino acid residues 403-410 from human TR alpha-1. This sequence is completely conserved between human and rat TR alpha-1.
Thyroid hormone receptors (TR), also known as c-erbA-1, are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1.
The corresponding gene for the thyroid hormone receptor apha-1 is NR1A1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 1; c-erb-A protein; c-erb-A thyroid hormone receptor; c-erbA-1; C-erbA-alpha; c-erbA-alpha2; c-erbAalpha; c-erbAalpha2; EAR-7; ERBA-related 7; Nuclear receptor subfamily 1 group A member 1; Thyroid hormone receptor alpha; thyroid hormone receptor alpha 1; thyroid hormone receptor alpha 1 (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 1 formerly ERBA1); thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian); thyroid normone nuclear receptor alpha variant 1; TR Alpha 1; TR alpha 2; triiodothyronine receptor; V-erbA-related protein 7
Gene Aliases: 6430529J03Rik; AR7; AW259572; c-ERBA-1; C-erba-alpha; CHNG6; EAR7; ERB-T-1; ERBA; ERBA1; NR1A1; Rvr; T3R[a]; T3Ralpha; THRA; THRA1; THRA2