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Invitrogen
THRA Polyclonal Antibody
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FIGURE: 1 / 12
THRA Antibody (PA1-211A) in ICC/IF
Immunofluorescent analysis of Thyroid Hormone Receptor alpha-1 (green) showing staining in the nucleus and cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Thyroid Hormone Receptor alpha-1 polyclonal antibody (Product # PA1-211A) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber... View More
Product Details
PA1-211A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Rat
Published species
C. elegans,
Human,
Mouse,
Non-human primate,
Rat,
Sheep,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: E(403) V F E D Q E V(410)
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum, PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-211A detects thyroid receptor (TR) from human, mouse, rat and chicken samples. This antibody does not detect TRv alpha -2 or TR beta-1.
PA1-211A has been successfully used in Western blot, IHC-P, ICC/IF and gel shift procedures. By Western blot, this antibody detects a ~48 kDa protein representing recombinant human TR alpha-1 expressed in E. coli. Gel shift experiments show that this product reacts with TR alpha-1 homodimers and TR alpha-1/retinoic X receptor (RXR) heterodimers.
PA1-211A immunizing peptide corresponds to amino acid residues 403-410 from human TR alpha-1. This sequence is completely conserved between human and rat TR alpha-1.
Target Information
The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 1; c-erb-A protein; c-erb-A thyroid hormone receptor; c-erbA-1; C-erbA-alpha; c-erbA-alpha2; c-erbAalpha; c-erbAalpha2; cerbA protein; cerbAalpha; E ERB-T-1; EAR-7; ERBA ALPHA; ERBA related 7; ERBA-related 7; MGC000261; MGC43240; Nuclear receptor subfamily 1 group A member 1; Oncogene ERBA; Thyroid hormone receptor alpha; thyroid hormone receptor alpha 1; thyroid hormone receptor alpha 1 (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 1 formerly ERBA1); thyroid hormone receptor beta; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian); thyroid normone nuclear receptor alpha variant 1; TR; TR alpha 1; TR alpha 1+2; TR alpha 2; triiodothyronine receptor; unnamed protein product; V-erbA-related protein 7
Gene Aliases: 6430529J03Rik; AR7; AW259572; c-ERBA-1; C-erba-alpha; CHNG6; EAR7; ERB-T-1; ERBA; ERBA1; NR1A1; Rvr; T3R[a]; T3Ralpha; THRA; THRA1; THRA2
UniProt ID: (Chicken) P04625, (Human) P10827, (Mouse) P63058, (Rat) P63059
Entrez Gene ID: (Chicken) 396251, (Human) 7067, (Mouse) 21833, (Rat) 81812
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
thyroid hormone binding
sequence-specific double-stranded DNA binding
transcription regulatory region sequence-specific DNA binding
steroid receptor RNA activator RNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
steroid hormone receptor activity
thyroid hormone receptor activity
transcription factor binding
TBP-class protein binding
protein domain specific binding
chromatin DNA binding
protein complex binding
protein homodimerization activity
transcription regulatory region DNA binding
intronic transcription regulatory region DNA binding
protein heterodimerization activity
DNA binding
single-stranded RNA binding
sequence-specific DNA binding
metal ion binding
protein dimerization activity
C4 zinc finger nuclear receptor
DNA-binding transcription factor
zinc finger transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
thyroid hormone mediated signaling pathway
hormone-mediated signaling pathway
cell differentiation
positive regulation of transcription from RNA polymerase II promoter
cartilage condensation
ossification
kidney development
liver development
regulation of thyroid hormone mediated signaling pathway
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
brain development
learning or memory
regulation of heart contraction
female courtship behavior
response to cold
positive regulation of myotube differentiation
negative regulation of RNA polymerase II transcriptional preinitiation complex assembly
erythrocyte differentiation
adrenal gland development
intracellular receptor signaling pathway
thyroid gland development
response to nutrient levels
regulation of myeloid cell apoptotic process
response to drug
cytoplasmic sequestering of transcription factor
steroid hormone mediated signaling pathway
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of transcription, DNA-templated
positive regulation of female receptivity
digestive tract development
embryonic organ development
regulation of lipid catabolic process
Type I pneumocyte differentiation
negative regulation of DNA-templated transcription, initiation
transcription, DNA-templated
regulation of transcription, DNA-templated
organ morphogenesis
lung development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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