Invitrogen

THRA/THRB Monoclonal Antibody (C3)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

16 Published Figures

23 References

View all (4) THRA/THRB antibodies

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Protocols

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THRA/THRB Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Thyroid Hormone Receptor in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Thyroid Hormone Receptor monoclonal antibody (Product # MA1-215) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Thyroid Hormone Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

THRA/THRB Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Thyroid Hormone Receptor showing staining in the cytoplasm and weak staining in the nucleus of paraffin-embedded mouse thyroid tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Thyroid Hormone Receptor Mouse Monoclonal Antibody (Product # MA1-215) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

THRA/THRB Antibody in Western Blot (WB) — Western blot of human thyroid hormone receptor using Product # MA1-215.

THRA/THRB Antibody in Western Blot, Immunoprecipitation (WB, IP) — Published figure using THRA/THRB monoclonal antibody (Product # MA1-215) in Western Blot and Immunoprecipitation

THRA/THRB Antibody in Western Blot, ChIP Assay (WB, ChIP) — Published figure using THRA/THRB monoclonal antibody (Product # MA1-215) in Western Blot and ChIP Assay

THRA/THRB Antibody in ChIP Assay (ChIP) — Fig. 3 Mkk6 -/- white adipose tissue is predisposed to T3-induced browning. a Activation of p38 and AMPK pathways in epididymal white fat from HFD-fed WT and Mkk6 -/- mice. Immunoblot analysis with the indicated antibodies was performed in extracts from mice starved overnight. b Immunoblot analysis of in vitro differentiated WT and Mkk6 -/- adipocytes. Representative from three different experiments done by duplicate. c Mkk6 -/ pre-adipocytes were infected with shRNA against AMPK, TAK1, TAB1, or scramble as a control. Activation of p38 was assayed by immunoblot of p38 and ATF2 phosphorylation. Representative from three different experiments. d UCP1 protein expression in in vitro differentiated WT and Mkk6 -/- adipocytes. Representative from three different experiments done by duplicate. e Mkk6 -/- pre-adipocytes were infected with shRNA against p38alpha or scramble as a control, and then differentiated to adipocytes. UCP1 levels were assayed by immunoblot n = 4. f Mitochondrial spare respiratory capacity (SRC) was assessed by Seahorse assay in primary WT and Mkk6 -/- adipocytes incubated with glucose oxidation (GO) or fatty acid oxidation (FAO) medium (mean +- SEM, WT n = 22 WT or 24 Mkk6 -/- wells from three mice cultured independently). g Representative transmission electronic microscopy images of mitochondria in eWAT from WT and Mkk6 -/- HFD-fed mice ( n = 3 mice). Scale bar: 500 nm. h Immunoblot analysis of UCP1 protein in in vitro differentiated WT and Mkk6 -/- adipocyte

THRA/THRB Antibody in Gel Shift (GS) — Published figure using THRA/THRB monoclonal antibody (Product # MA1-215) in Gel Shift

THRA/THRB Antibody — Figure 2 (a) The amino acid sequence of the DNA-binding domain of TR-beta. The boxed region outlines the DNA recognition alpha-helix. The shaded circles indicate the P-box amino acids. The exchange of glutamic acid 125 and glycine 126 to glycine and serine in the GS125 mutation is indicated. (b) Liver RNA was amplified by RT-PCR using primers located in sequences corresponding to exons 2 and 4 (A-1, A-2) and exons 3 and 4 (B-1, B-2), which are indicated by arrows. (c) RT-PCR results from liver RNA using the indicate primers. A 445-bp fragment from WT (+/+) and TR-beta GS/GS animals was observed with the A primer set. A short fragment (344 bp) from the A primer set and no band from the B set were obtained from RNA transcripts from TR-beta-/- mice. (d) DNA sequence of the 445-bp RT-PCR fragment. The two mutated amino acids are indicated. The deleted mRNA from TR-beta-/- encoded a putative peptide that terminated after eight bases in exon 4 at a TAG stop codon. This was confirmed by sequencing the 344-bp fragment from TR-beta-/- mice (data not shown). (e) Western blot analysis of liver total cellular protein extracts from WT, TR-beta-/-, and TR-beta GS/GS animals (a C-terminal TR-beta monoclonal antibody was used), indicating that the GS125 mutation did not affect expression from the TR-beta locus. {KO}

Product Details

MA1-215

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 11 publications 11 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20	-
Immunocytochemistry (ICC/IF)	1:20	View 1 publication 1 publication
Immunoprecipitation (IP)	Assay-dependent	View 3 publications 3 publications
ChIP assay (ChIP)	-	View 5 publications 5 publications
Gel Shift (GS)	Assay-dependent	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human
Published species	Hamster, Human, Mouse, Non-human primate, Rat, Xenopus
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	C3
Immunogen	Purified fragment of human TR beta-1 corresponding to residues 201-456.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	ascites
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_325420

Product Specific Information

MA1-215 detects thyroid hormone receptor (TR) from human tissues. This antibody recognizes TR beta-1 and also cross-reacts with TR alpha-1.

MA1-215 has been successfully used in Western blot, IF, immunoprecipitation, and gel shift procedures. By Western blot, this antibody detects a 55 and a 52 kDa protein representing recombinant human TR beta-1 expressed in E. coli. MA1-215 immunoprecipitates 52 kDa and 55 kDa proteins, representing TR beta-1 and a 48 kDa protein representing TR alpha-1 expressed in E. coli.

The MA1-215 immunogen is a purified fragment of human TR beta-1 corresponding to amino acid residues 201-456. This antibody recognizes an epitope in the hormone binding domain of hTR beta-1, amino acid residues 235-414. This sequence is conserved in hTR alpha-1.

Target Information

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Extracellular histones, a new class of inhibitory molecules of CNS axonal regeneration.

Brain communications

Siddiq MM,Hannila SS,Zorina Y,Nikulina E,Rabinovich V,Hou J,Huq R,Richman EL,Tolentino RE,Hansen J,Velenosi A,Kwon BK,Tsirka SE,Maze I,Sebra R,Beaumont KG,Toro CA,Cardozo CP,Iyengar R,Filbin MT

MA1-215 was used in Protein Assay to identify extracellular histones as a new class of nerve regeneration-inhibiting molecules within the injured CNS.

Sat Jan 08 00:00:00 EST 2022

Early life variations in temperature exposure affect the epigenetic regulation of the paraventricular nucleus in female rat pups.

Proceedings. Biological sciences

Lauby SC,McGowan PO

MA1-215 was used in Chromatin immunoprecipitation to suggest that repeated room temperature exposure may affect DNA methylation levels as a consequence of alterations in thyroid hormone receptor signalling.

Wed Oct 28 00:00:00 EDT 2020

The protective variant rs7173049 at LOXL1 locus impacts on retinoic acid signaling pathway in pseudoexfoliation syndrome.

Human molecular genetics

Berner D,Hoja U,Zenkel M,Ross JJ,Uebe S,Paoli D,Frezzotti P,Rautenbach RM,Ziskind A,Williams SE,Carmichael TR,Ramsay M,Topouzis F,Chatzikyriakidou A,Lambropoulos A,Sundaresan P,Ayub H,Akhtar F,Qamar R,Zenteno JC,Cruz-Aguilar M,Astakhov YS,Dubina M,Wiggs J,Ozaki M,Kruse FE,Aung T,Reis A,Khor CC,Pasutto F,Schlötzer-Schrehardt U

MA1-215 was used in Electrophoretic mobility shift assay to determine the biological importance of pseudoexfoliation - associated common variants.

Thu Aug 01 00:00:00 EDT 2019

Reversing thyroid-hormone-mediated repression of a HSV-1 promoter via computationally guided mutagenesis.

Journal of cell science

Figliozzi RW,Chen F,Hsia SV

MA1-215 was used in ChIP assay to elucidate the impact on transcriptional repression of nucleotide sequence or orientation within thyroid hormone DNA-binding nuclear receptor binding sites.

Wed Nov 01 00:00:00 EDT 2017

MKK6 controls T3-mediated browning of white adipose tissue.

Nature communications

Matesanz N,Bernardo E,Acín-Pérez R,Manieri E,Pérez-Sieira S,Hernández-Cosido L,Montalvo-Romeral V,Mora A,Rodríguez E,Leiva-Vega L,Lechuga-Vieco AV,Ruiz-Cabello J,Torres JL,Crespo-Ruiz M,Centeno F,Álvarez CV,Marcos M,Enríquez JA,Nogueiras R,Sabio G

MA1-215 was used in Chromatin immunoprecipitation to demonstrate that MKK6 deletion in adipocytes increases white adipose tissue thermogenic capacity and this is a potential therapeutic strategy for obesity.

Wed Oct 11 00:00:00 EDT 2017

Thyroid hormone-dependent epigenetic suppression of herpes simplex virus-1 gene expression and viral replication in differentiated neuroendocrine cells.

Journal of the neurological sciences

Figliozzi RW,Chen F,Balish M,Ajavon A,Hsia SV

MA1-215 was used in Western Blotting and ChIP to investigate whether triiodothyronine treatment of infected differentiated neuron-like cells reduces the ability of HSV-1 to produce viral progeny.

Sat Nov 15 00:00:00 EST 2014

Thyroid hormone-dependent epigenetic suppression of herpes simplex virus-1 gene expression and viral replication in differentiated neuroendocrine cells.

Journal of the neurological sciences

Figliozzi RW,Chen F,Balish M,Ajavon A,Hsia SV

MA1-215 was used in Western Blotting and ChIP to investigate whether triiodothyronine treatment of infected differentiated neuron-like cells reduces the ability of HSV-1 to produce viral progeny.

Sat Nov 15 00:00:00 EST 2014

The p160 coactivator PAS-B motif stabilizes nuclear receptor binding and contributes to isoform-specific regulation by thyroid hormone receptors.

The Journal of biological chemistry

Privalsky ML,Lee S,Hahm JB,Young BM,Fong RN,Chan IH

MA1-215 was used in western blot to investigate the relationship between p160 coactivators and thyroid hormone receptors.

Fri Jul 17 00:00:00 EDT 2009

Inhibition of apoptotic potency by ligand stimulated thyroid hormone receptors located in mitochondria.

Apoptosis : an international journal on programmed cell death

Saelim N,Holstein D,Chocron ES,Camacho P,Lechleiter JD

MA1-215 was used in western blot to investigate the role of thyroid hormone receptors in apoptosis

Mon Oct 01 00:00:00 EDT 2007

The rat thyroid hormone receptor (TR) Deltabeta3 displays cell-, TR isoform-, and thyroid hormone response element-specific actions.

Endocrinology

Harvey CB,Bassett JH,Maruvada P,Yen PM,Williams GR

MA1-215 was used in western blot to investigate the mechanism for rat thyroid hormone receptor Deltabeta3 actions.

Sun Apr 01 00:00:00 EDT 2007

Rapid signaling at the plasma membrane by a nuclear receptor for thyroid hormone.

Proceedings of the National Academy of Sciences of the United States of America

Storey NM,Gentile S,Ullah H,Russo A,Muessel M,Erxleben C,Armstrong DL

MA1-215 was used in immunoprecipitation to investigate the molecular mechanism for the effects of thyroid hormone on human brain development.

Tue Mar 28 00:00:00 EST 2006

Rapid signaling at the plasma membrane by a nuclear receptor for thyroid hormone.

Proceedings of the National Academy of Sciences of the United States of America

Storey NM,Gentile S,Ullah H,Russo A,Muessel M,Erxleben C,Armstrong DL

MA1-215 was used in immunoprecipitation to investigate the molecular mechanism for the effects of thyroid hormone on human brain development.

Tue Mar 28 00:00:00 EST 2006

Alternative mRNA splicing of SMRT creates functional diversity by generating corepressor isoforms with different affinities for different nuclear receptors.

The Journal of biological chemistry

Goodson ML,Jonas BA,Privalsky ML

MA1-215 was used in EMSA to investigate the alternative mRNA splicing of silencing mediator of retinoic acid and thyroid hormone receptors.

Fri Mar 04 00:00:00 EST 2005

Alternative mRNA splicing of SMRT creates functional diversity by generating corepressor isoforms with different affinities for different nuclear receptors.

The Journal of biological chemistry

Goodson ML,Jonas BA,Privalsky ML

MA1-215 was used in EMSA to investigate the alternative mRNA splicing of silencing mediator of retinoic acid and thyroid hormone receptors.

Fri Mar 04 00:00:00 EST 2005

Nontranscriptional modulation of intracellular Ca2+ signaling by ligand stimulated thyroid hormone receptor.

The Journal of cell biology

Saelim N,John LM,Wu J,Park JS,Bai Y,Camacho P,Lechleiter JD

MA1-215 was used in immunoprecipitation and western blot to study the regulation of IP3-mediated calcium signaling by thyroid hormone receptors.

Mon Dec 06 00:00:00 EST 2004

Nontranscriptional modulation of intracellular Ca2+ signaling by ligand stimulated thyroid hormone receptor.

The Journal of cell biology

Saelim N,John LM,Wu J,Park JS,Bai Y,Camacho P,Lechleiter JD

MA1-215 was used in immunoprecipitation and western blot to study the regulation of IP3-mediated calcium signaling by thyroid hormone receptors.

Mon Dec 06 00:00:00 EST 2004

Multiple messenger ribonucleic acid variants regulate cell-specific expression of human thyroid hormone receptor beta1.

Molecular endocrinology (Baltimore, Md.)

Frankton S,Harvey CB,Gleason LM,Fadel A,Williams GR

MA1-215 was used in immunocytochemistry to demonstrate the function of human thyroid hormone receptor beta1 5-UTR mRNAs.

Thu Jul 01 00:00:00 EDT 2004

Thyroid-hormone-dependent negative regulation of thyrotropin beta gene by thyroid hormone receptors: study with a new experimental system using CV1 cells.

The Biochemical journal

Nakano K,Matsushita A,Sasaki S,Misawa H,Nishiyama K,Kashiwabara Y,Nakamura H

MA1-215 was used in western blot to investigate the role of thyroid hormone receptors in regulation of thyrotropin beta gene

Mon Mar 01 00:00:00 EST 2004

Thyroid hormone action in the absence of thyroid hormone receptor DNA-binding in vivo.

The Journal of clinical investigation

Shibusawa N,Hashimoto K,Nikrodhanond AA,Liberman MC,Applebury ML,Liao XH,Robbins JT,Refetoff S,Cohen RN,Wondisford FE

MA1-215 was used in western blot to investigate the possible role of DNA-binding in thyroid hormone's nuclear functions

Fri Aug 01 00:00:00 EDT 2003

Thyroid hormone receptor DNA binding is required for both positive and negative gene regulation.

The Journal of biological chemistry

Shibusawa N,Hollenberg AN,Wondisford FE

Published figure using THRA/THRB monoclonal antibody (Product # MA1-215) in ChIP assay

Fri Jan 10 00:00:00 EST 2003

Thyroid hormone receptor DNA binding is required for both positive and negative gene regulation.

The Journal of biological chemistry

Shibusawa N,Hollenberg AN,Wondisford FE

Published figure using THRA/THRB monoclonal antibody (Product # MA1-215) in ChIP assay

Fri Jan 10 00:00:00 EST 2003

Ligand-dependent degradation of retinoid X receptors does not require transcriptional activity or coactivator interactions.

Molecular and cellular biology

Osburn DL,Shao G,Seidel HM,Schulman IG

MA1-215 was used in western blot to study the agonist-stimulated RXR degradation

Wed Aug 01 00:00:00 EDT 2001

Cloning and characterization of two novel thyroid hormone receptor beta isoforms.

Molecular and cellular biology

Williams GR

MA1-215 was used in western blot to characterize two novel isoforms of thyroid hormone beta receptor.

Wed Nov 01 00:00:00 EST 2000

Conformational changes of human beta 1 thyroid hormone receptor induced by binding of 3,3',5-triiodo-L-thyronine.

Biochemical and biophysical research communications

Bhat MK,Parkison C,McPhie P,Liang CM,Cheng SY

MA1-215 was used in immunoprecipitation to perform structural analysis of human thyroid hormone receptor beta 1

Tue Aug 31 00:00:00 EDT 1993

Bioinformatics

Protein Aliases: c-erbA-1; c-erbA-2; c-erbA-alpha; c-erbA-beta; EAR-7; ERBA-related 7; Nuclear receptor subfamily 1 group A member 1; Nuclear receptor subfamily 1 group A member 2; oncogene ERBA2; THR; thyroid hormone nuclear receptor beta variant 1; Thyroid hormone receptor alpha; thyroid hormone receptor alpha 1; Thyroid hormone receptor beta; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian); thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian); thyroid normone nuclear receptor alpha variant 1; triiodothyronine receptor; V-erbA-related protein 7

Gene Aliases: AR7; c-ERBA-1; C-ERBA-2; C-ERBA-BETA; CHNG6; EAR7; ERB-T-1; ERBA; ERBA1; ERBA2; GRTH; NR1A1; NR1A2; PRTH; THR1; THRA; THRA1; THRA2; THRB; THRB1; THRB2

UniProt ID: (Human) P10827, (Human) P10828

Entrez Gene ID: (Human) 7067, (Human) 7068

Molecular Function: C4 zinc finger nuclear receptor DNA-binding transcription factor zinc finger transcription factor gene-specific transcriptional regulator