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THRAP5 Antibody (PA5-116039) in IHC (P)
Immunohistochemistry analysis of THRAP5 in mouse lung tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-116039) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.
Product Details
PA5-116039
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MED16(Accession Q9Y2X0), corresponding to amino acid residues Q619-K669.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900673
Product Specific Information
Antibody detects endogenous levels of total THRAP5.
Target Information
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DRIP92; Mediator complex subunit 16; Mediator of RNA polymerase II transcription subunit 16; thyroid hormone receptor associated protein 5; Thyroid hormone receptor-associated protein 5; thyroid hormone receptor-associated protein 95; Thyroid hormone receptor-associated protein complex 95 kDa component; thyroid hormone receptor-associated protein, 95-kD subunit; Trap95; Vitamin D3 receptor-interacting protein complex 92 kDa component
Gene Aliases: 95kDa; A630083L04; DRIP92; MED16; THRAP5; TRAP95
UniProt ID: (Human) Q9Y2X0
Entrez Gene ID: (Human) 10025, (Rat) 299607, (Mouse) 216154
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
intracellular steroid hormone receptor signaling pathway
androgen receptor signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of receptor activity
biological_process
transcription, DNA-templated
regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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