PA1-213A detects thyroid hormone receptor (TR) beta-1 from human and rat tissues. This antibody does not detect TR alpha-1 or TRv alpha-2.
PA1-213A has been successfully used in Western blot, immunohistochemistry (paraffin), IF and gel shift procedures. By Western blot, this antibody detects a ~55 kDa protein representing recombinant human TRv beta-1 expressed in E. coli.
PA1-213A immunizing peptide corresponds to residues 62-81 of human TR beta-1, with an N-terminal added cysteine. Alternative numbering of this protein has this peptide corresponding to residues 67-86 of human TR beta-1. This sequence is completely conserved between human and rat TR beta-1.
Thyroid hormone receptors (TR), also known as c-erbA-2, are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1.
The corresponding gene for the thyroid hormone receptor beta-1 is NR1A2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: c-erbA-2; c-erbA-beta; Nuclear receptor subfamily 1 group A member 2; oncogene ERBA2; thyroid hormone nuclear receptor beta variant 1; Thyroid hormone receptor beta; thyroid hormone receptor beta 3; thyroid hormone receptor beta2delta; thyroid hormone receptor, beta (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2); thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian); TR beta 1; TRbeta2Delta
Gene Aliases: C-ERBA-2; C-ERBA-BETA; ERBA2; GRTH; NR1A2; PRTH; RATT3REC; T3rec; THR1; THRB; THRB1; THRB2; TRbeta
UniProt ID: (Human) P10828