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Invitrogen
THRB Polyclonal Antibody
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FIGURE: 1 / 5
THRB Antibody (PA5-29684) in ICC/IF
Immunofluorescence analysis of THRB was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with THRB Polyclonal Antibody (Product # PA5-29684) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A3279... View More
Product Details
PA5-29684
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Thyroid Hormone Receptor beta. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.76 mg/mL
Amount
76 µg
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Predicted reactivity: Mouse (93%), Rat (92%), Sheep (90%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Thyroid hormone receptors (TR), also known as c-erbA-2, are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1. The corresponding gene for the thyroid hormone receptor beta-1 is NR1A2.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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