Invitrogen

TID1 Polyclonal Antibody

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Product Details

PA5-29225

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human TID1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2546701

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.

Predicted reactivity: Mouse (94%), Rat (93%), Xenopus laevis (80%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TID1 is a human homolog of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l(2)tid and encodes two mitochondrial matrix localized splice variants of human Tid-1 designated hTid-1S and hTid-1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperones for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid-1L increases apoptosis induced by DNA damaging agents as mitomycin-C and TNF-a. A J-domain mutant of hTid-1L can dominantly suppress apoptosis and in sharp contrast the J-domain mutant of hTid-1S increases apoptosis. Expression of hTid-1S and hTid-1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti- and pro- apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DnaJ (Hsp40) homolog, subfamily A, member 3; DnaJ homolog subfamily A member 3, mitochondrial; DnaJ protein Tid-1; Hepatocellular carcinoma-associated antigen 57; hTid-1; Tumorous imaginal discs protein Tid56 homolog

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Gene Aliases: DNAJA3; HCA57; hTID-1; Tid-1; TID1

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UniProt ID: (Human) Q96EY1

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Entrez Gene ID: (Human) 9093, (Rat) 360481

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Function(s)
protein binding ATP binding Hsp70 protein binding GTPase regulator activity receptor tyrosine kinase binding metal ion binding unfolded protein binding interferon-gamma receptor binding transcription factor binding protein kinase binding NF-kappaB binding chaperone binding chaperone
Process(es)
negative regulation of transcription from RNA polymerase II promoter mitochondrial DNA replication protein folding activation-induced cell death of T cells mitochondrion organization small GTPase mediated signal transduction cell aging negative regulation of cell proliferation response to heat T cell differentiation in thymus positive regulation of T cell proliferation negative regulation of programmed cell death regulation of catalytic activity skeletal muscle acetylcholine-gated channel clustering negative regulation of protein kinase activity apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process neuromuscular junction development positive regulation of protein ubiquitination negative regulation of NF-kappaB transcription factor activity response to interferon-gamma protein refolding regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein stabilization negative regulation of interferon-gamma-mediated signaling pathway
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