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Invitrogen

TID1 Polyclonal Antibody

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TID1 Polyclonal Antibody

Product Details

PA5-96554

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 221-480 of human DNAJA3 (NP_0051383)
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Amount

40 µg

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2808356

Product Specific Information

Immunogen sequence: AKGVNKEFTV NIMDTCERCN GKGNEPGTKV QHCHYCGGSG METINTGPFV MRSTCRRCGG RGSIIISPCV VCRGAGQAKQ KKRVMIPVPA GVEDGQTVRM PVGKREIFIT FRVQKSPVFR RDGADIHSDL FISIAQALLG GTARAQGLYE TINVTIPPGT QTDQKIRMGG KGIPRINSYG YGDHYIHIKI RVPKRLTSRQ QSLILSYAED ETDVEGTVNG VTLTSSGGST MDSSAGSKAR REAGEDEEGF LSKLKKMFTS; Positive Samples: BT-474, Jurkat, 293T, HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver; Cellular Location: Cell junction, Cytoplasm, Mitochondrion matrix, Peripheral membrane protein, cytosol, postsynaptic cell membrane, synapse

Target Information

TID1 is a human homolog of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l(2)tid and encodes two mitochondrial matrix localized splice variants of human Tid-1 designated hTid-1S and hTid-1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperones for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid-1L increases apoptosis induced by DNA damaging agents as mitomycin-C and TNF-a. A J-domain mutant of hTid-1L can dominantly suppress apoptosis and in sharp contrast the J-domain mutant of hTid-1S increases apoptosis. Expression of hTid-1S and hTid-1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti- and pro- apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DnaJ (Hsp40) homolog, subfamily A, member 3; DnaJ homolog subfamily A member 3, mitochondrial; DnaJ protein Tid-1; Hepatocellular carcinoma-associated antigen 57; hTid-1; hTid-1 protein; mTid-1; Tumorous imaginal discs protein Tid56 homolog; unnamed protein product

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Gene Aliases: 1200003J13Rik; 1810053A11Rik; C81173; DNAJA3; HCA57; hTID-1; Tid-1; TID1; Tid1-L; Tid1-S; Tid1l

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UniProt ID: (Human) Q96EY1, (Mouse) Q99M87

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Entrez Gene ID: (Human) 9093, (Mouse) 83945, (Rat) 360481

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Function(s)
receptor binding interferon-gamma receptor binding protein binding ATP binding zinc ion binding protein kinase binding Hsp70 protein binding GTPase regulator activity heat shock protein binding macromolecular complex binding metal ion binding NF-kappaB binding unfolded protein binding RNA polymerase II sequence-specific DNA binding transcription factor binding IkappaB kinase complex binding DNA-binding transcription factor binding transcription factor binding receptor tyrosine kinase binding chaperone binding chaperone
Process(es)
negative regulation of transcription from RNA polymerase II promoter immune system process regulation of immune system process mitochondrial DNA replication protein folding apoptotic process activation-induced cell death of T cells response to stress mitochondrion organization small GTPase mediated signal transduction neuromuscular junction development negative regulation of cell proliferation response to heat positive regulation of protein ubiquitination T cell differentiation in thymus response to interferon-gamma positive regulation of T cell proliferation regulation of cell proliferation positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of programmed cell death negative regulation of I-kappaB kinase/NF-kappaB signaling protein stabilization negative regulation of interferon-gamma-mediated signaling pathway skeletal muscle acetylcholine-gated channel clustering cellular senescence negative regulation of protein kinase activity activation of cysteine-type endopeptidase activity involved in apoptotic process cell aging embryo development negative regulation of NF-kappaB transcription factor activity protein refolding regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of catalytic activity
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