|Tested species reactivity||Dog, Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 220-270 of human TIGAR.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TIGAR is a p53-inducible protein that functions to regulate glycolysis and protect against oxidative stress and is part of the p53 tumor suppressor pathway. It encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2, 6-bisphosphate. TIGAR functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Overexpression of TIGAR in mouse and human cells resulted in inhibition of glycolysis, as well as a decrease in intracellular reactive oxygen species (ROS) and provides some protection from DNA damage-induced apoptosis in a cell type-specific manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.