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Invitrogen
TIGAR Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
TIGAR Antibody (PA5-85436) in WB
Western blot was performed using Anti-TIGAR Polyclonal Antibody (Product # PA5-85436) and a 30 kDa band corresponding to Fructose-2,6-bisphosphatase TIGAR was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the prima... View More
Product Details
PA5-85436
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human TIGAR.
Conjugate
Form
Liquid
Concentration
0.71 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792576
Product Specific Information
Keep as concentrated solution.^M
^M
Predicted reactivity: Bovine (80%).^M
^M
Positive Control: Raji.^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a member of the kelch family of proteins, which are characterized by kelch repeat motifs and a POZ/BTB protein-binding domain. It is thought that kelch repeats are actin binding domains. However, the specific function of this protein has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Fructose-2,6-bisphosphatase TIGAR; fructose-2,6-bisphosphate 2-phosphatase; probable fructose-2,6-bisphosphatase TIGAR; TP53-induced glycolysis and apoptosis regulator; TP53-induced glycolysis regulatory phosphatase; transactivated by NS3TP2 protein
Gene Aliases: C12orf5; FR2BP; TIGAR
UniProt ID: (Human) Q9NQ88
Entrez Gene ID: (Human) 57103
response to ischemia
fructose 2,6-bisphosphate metabolic process
autophagy
apoptotic process
cellular response to DNA damage stimulus
response to xenobiotic stimulus
response to gamma radiation
positive regulation of cardiac muscle cell apoptotic process
dephosphorylation
fructose 1,6-bisphosphate metabolic process
negative regulation of programmed cell death
regulation of pentose-phosphate shunt
positive regulation of DNA repair
negative regulation of glycolytic process
intestinal epithelial cell development
cellular response to cobalt ion
cellular response to hypoxia
negative regulation of neuron death
negative regulation of macromitophagy
regulation of response to DNA damage checkpoint signaling
positive regulation of hexokinase activity
negative regulation of glucose catabolic process to lactate via pyruvate
negative regulation of reactive oxygen species metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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