Western blot analysis of TIGD1 using A) 30 µg 293T whole cell extract and B) 30 µg whole cell extract of human TIGD1-transfected 293T cells. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a TIGD1 polyclonal antibody (Product # PA5-28642) at a dilution of 1:5000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 530 and 591 of Human TIGD1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28642 targets TIGD1 in WB applications and shows reactivity with Human samples.
The PA5-28642 immunogen is synthetic peptide corresponding to a region within amino acids 530 and 591 of Human TIGD1.
The protein encoded by this gene belongs to the tigger subfamily of the pogo superfamily of DNA-mediated transposons in humans. These proteins are related to DNA transposons found in fungi and nematodes, and more distantly to the Tc1 and mariner transposases. They are also very similar to the major mammalian centromere protein B. The exact function of this gene is not known.
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