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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TIMELESS Antibodies

          Invitrogen

          TIMELESS Polyclonal Antibody

          View all (17) TIMELESS antibodies

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          Cite TIMELESS Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          TIMELESS Antibody in Immunocytochemistry (ICC/IF)
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          TIMELESS Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          TIMELESS Antibody (PA5-28026) in ICC/IF

          Immunocytochemistry analysis of TIMELESS in paraformaldehyde-fixed HeLa. Samples were incubated with TIMELESS polyclonal antibody (Product # PA5-28026) using a dilution of 1:500. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          TIMELESS Antibody in Immunocytochemistry (ICC/IF)
          TIMELESS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TIMELESS Antibody in Western Blot (WB)
          TIMELESS Polyclonal Antibody

          Product Details

          PA5-28026

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the N-terminus region of human TIMELESS. The exact sequence is proprietary.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.1M tris glycine, pH 7, with 10% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2545502

          Product Specific Information

          Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Raji.

          Predicted reactivity: Mouse (90%), Rat (90%), Sheep (93%), Bovine (93%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          The general transcription factor IIE (TFIIE) is part of the RNA polymerase II transcription initiation complex, recruiting TFIIH and being essential for promoter clearance by RNA polymerase II. TFIIE is a heterodimer (and sometimes heterotetramer) of alpha and beta subunits. The protein encoded by this gene represents the beta subunit of TFIIE.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

          Have you cited this product in a publication?
          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: hTIM; Protein timeless homolog; timeless circadian clock 1; timeless homolog; Tof1 homolog

          View more View less

          Gene Aliases: hTIM; TIM; TIM1; TIMELESS; TIMELESS1

          View more View less

          UniProt ID: (Human) Q9UNS1

          View more View less

          Entrez Gene ID: (Human) 8914

          View more View less

          Function(s)
          protein binding protein homodimerization activity protein heterodimerization activity
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter morphogenesis of an epithelium DNA replication transcription, DNA-templated cellular response to DNA damage stimulus mitotic nuclear division circadian rhythm detection of abiotic stimulus response to abiotic stimulus lung development regulation of cell proliferation regulation of circadian rhythm cell cycle phase transition negative regulation of transcription, DNA-templated branching morphogenesis of an epithelial tube cell division
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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