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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the first loop domain of human TIMP1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25310 detects TIMP1 from human samples. This antibody does not cross-react with other TIMP family members..
PA1-25310 has been successfully used in immunohistochemistry (frozen) immunoprecipitation, and Western blot procedures. By Western blot, this antibody detects a 29 kDa band from reduced samples. Please note that TIMP1 is expressed in low (pg/ml) levels in most cell types. Treatment of cells with a phorbol ester (ie. PMA) stimulates production fo TIMP1 in some cell types, but concentration of cell culture media may still be necessary to visualize bands in WB.
The PA1-25310 immunogen is a synthetic peptide corresponding to the first loop domain of human TIMP1.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; metalloproteinase inhibitor 1; TIMP-1; tissue inhibitor of metalloproteinases 1
CLGI; EPA; EPO; HCI; RP1-230G1.3; TIMP