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PA1-25310 detects TIMP1 from human samples. This antibody does not cross-react with other TIMP family members..
PA1-25310 has been successfully used in immunohistochemistry (frozen) immunoprecipitation, and Western blot procedures. By Western blot, this antibody detects a 29 kDa band from reduced samples. Please note that TIMP1 is expressed in low (pg/ml) levels in most cell types. Treatment of cells with a phorbol ester (ie. PMA) stimulates production fo TIMP1 in some cell types, but concentration of cell culture media may still be necessary to visualize bands in WB.
The PA1-25310 immunogen is a synthetic peptide corresponding to the first loop domain of human TIMP1.
Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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