Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
TIMP4 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 2
TIMP4 Antibody (PA5-92959) in WB
Western blot analysis of TIMP4 in extracts of various cell lines. Samples were incubated with TIMP4 Polyclonal antibody (Product # PA5-92959) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-92959
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
0.54 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: HT-29, Mouse heart, Mouse brain, Mouse ovary, Rat heart, Rat brain
Target Information
Tissue inhibitor of metalloproteinase 4 (TIMP4) is encoded by the gene TIMP4 located on chromosome 3. It belongs to the family of TIMP genes and is known to inhibit the activity of matrix metalloproteinases. Matrix metalloproteinases (MMP) are a group of proteinases that degrade the components of extracellular matrix. TIMP4 irreversibly binds to MMPs and inactivates them with the help of zinc as a cofactor. The netrin domain of TIMP4 is involved in platelet recruitment and aggregation. Other biological functions include response to cytokine and peptide hormone stimuli and involvement with the ovulation cycle and CNS development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Metalloproteinase inhibitor 4; TIMP 4; TIMP-4; tissue inhibitor of metalloproteinase 4; Tissue inhibitor of metalloproteinases 4
Gene Aliases: TIMP-4; TIMP4
UniProt ID: (Human) Q99727, (Mouse) Q9JHB3, (Rat) P81556
Entrez Gene ID: (Human) 7079, (Mouse) 110595, (Rat) 680130
Notch signaling pathway
central nervous system development
biological_process
response to organic substance
negative regulation of endopeptidase activity
response to lipopolysaccharide
response to cytokine
response to drug
ovulation cycle
negative regulation of catalytic activity
response to peptide hormone
negative regulation of membrane protein ectodomain proteolysis
response to hormone
negative regulation of peptidase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support