|Tested species reactivity||Human, Mouse, Non-human primate, Pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||C-terminus (within residues 350-435) of the human TIP47 protein. [Swiss Prot #O60664]|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western Blot analysis, a band is seen at ~47 kDa, representing isoform B.
In some samples, a ~28 kDa band may be observed which represents a splice isoform.
Suggested positive control: antigen standard for M6PRBP1 (transient overexpression lysate), HeLa whole cell lysate and 3T3 L1 lysate.
TIP47 has been described as cargo protein involved in the trafficking of the mannose-6-phosphate receptor between endosomes and the Golgi complex. Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs and is required for endosome to Golgi transport. This protein also binds directly to the GTPase RAB9, a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. TIP47 has been localized in milk fat globule membranes of human and bovine origin. It has been described as a placental protein, as well. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. Therefore, this protein is probably significant as an oncodevelopmental marker.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.