|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to a region within the N-terminus (within residues 1-100) of the mouse TIP47 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western Blot analysis, a band is seen ~47 kDa (isoform B, containing the first 184 residues that isoform A is lacking).
Suggested positive control: HeLa whole cell lysate and 3T3 L1 lysate.
TIP47 (tail-interacting protein 47) is a member of PAT family of proteins (perilipin, ADRP, S3-12 and OXPAT) which plays a key role in the packaging/storage of neutral lipids and is required for mannose 6-phosphate receptors (M6PR) transport from endosomes to trans-Golgi network (TGN). TIP47 localizes in cytoplasm, endosome membrane as peripheral membrane protein towards cytoplasmic side, core of lipid bodies/ lipid droplet, in lipid sails and in the envelope, and it interacts with M6PR, IGF2R etc. via the cytoplasmic domain. As a cargo protein, TIP47 facilitates the delivery of M6PR from endosomes to TGN where it binds to cytoplasmic domains of cation-independent/-dependent M6PR and translocates to late endosomes by binding to Rab9 GTPase. Like other PAT family members, TIP47 associates with lipid droplets and influence insulin signaling as evidenced by combined knockout of ADRP /TIP47 leading to decreased insulin sensitivity. During HIV-1 infection, TIP47 acts as a cellular cofactor playing a part in Env incorporation, allowing the encounter and physical association between HIV-1 Gag and Env proteins during viral assembly process. TIP47 is identical to pregnancy-related placental tissue protein 17b (PP17b) and is overexpressed in cervical carcinoma patients wherein it is considered a serum tumor marker.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.