|Tested species reactivity||Human, Rat|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 14 amino acid peptide from near the carboxy terminus of human TIP47.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||10 µg/ml|
|Western Blot (WB)||0.5-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is Daudi cell lysate.
PA5-20272 can be used with blocking peptide PEP-0387.
Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal surface by the Ras-associated protein Rab9 GTPase, enabling it to bind more efficiently to MPR cytoplasmic domains. Recently, it was shown that TIP47 regulates the expression of Rab9, as expression of siRNA to TIP47 in transfected cells dramatically decreased the half-life of the Rab9 protein in addition to stabilizing the subcellular localization of Rab9. At least two isoforms of TIP47 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Real-Time Tracking of BODIPY-C12 Long-Chain Fatty Acid in Human Term Placenta Reveals Unique Lipid Dynamics in Cytotrophoblast Cells.
PA5-20272 was used in immunocytochemistry to discover the unique lipid dynamics in cytotrophoblast cells due to real-time tracking of BODIPY-C12 long-chain fatty acid in human term placenta
|Kolahi K,Louey S,Varlamov O,Thornburg K||PloS one (11:null)||2016|
Myotubes from severely obese type 2 diabetic subjects accumulate less lipids and show higher lipolytic rate than myotubes from severely obese non-diabetic subjects.
PA5-20272 was used in western blot to study the role of skeletal muscle lipid handling in obese patients with type 2 diabetes
|Bakke SS,Feng YZ,Nikoli¿ N,Kase ET,Moro C,Stensrud C,Damlien L,Ludahl MO,Sandbu R,Solheim BM,Rustan AC,Hjelmesæth J,Thoresen GH,Aas V||PloS one (10:null)||2015|