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Invitrogen
TIP60 Polyclonal Antibody
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FIGURE: 1 / 5
TIP60 Antibody (PA5-34548) in ICC/IF
Immunofluorescence analysis of TIP60 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-TIP60 Rabbit Polyclonal Antibody (Product # PA5-34548) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (P... View More
Product Details
PA5-34548
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 15 amino acid peptide near the carboxy terminus of human KAT5
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is human brain tissue lysate.
PA5-34548 can be used with blocking peptide PEP-1591.
Target Information
TIP60 is a transcription factor, originally isolated as an HIV-1 TAT-interactive protein, known to interact with the viral TAT protein that leads to HTATIP polyubiquitination and targets it for degradation. It belongs to the MYST family of histone acetyl transferases (HATs). TIP60 is a catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. A co-regulator of transcription factors, TIP60 either promotes or suppresses tumorigenesis. TIP60 is an enzyme that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 60 kDa Tat-interactive protein; cPLA(2)-interacting protein; cPLA2 interacting protein; Histone acetyltransferase HTATIP; Histone acetyltransferase KAT5; HIV-1 Tat interactive protein; HIV-1 tat interactive protein 1, 60 kDa homolog; HIV-1 tat interactive protein 1, homolog; HIV-1 Tat interactive protein 60 kD; HIV-1 Tat interactive protein, 60 kD; HIV-1 Tat interactive protein, 60kDa; HIV-1 tat interactive protein, homolog; K(lysine) acetyltransferase 5; K-acetyltransferase 5; Lysine acetyltransferase 5; Protein 2-hydroxyisobutyryltransferase KAT5; Protein acetyltransferase KAT5; Protein crotonyltransferase KAT5; Protein lactyltransferase KAT5; Tat interacting protein, 60kDa; Tat-interactive protein-60; Tip60; TIP60(HTATIP)
Gene Aliases: AI839539; CPLA2; ESA1; HTATIP; HTATIP1; KAT5; PLIP; TIP; Tip55; TIP60; Tip60b; ZC2HC5
UniProt ID: (Human) Q92993, (Mouse) Q8CHK4, (Rat) Q99MK2
Entrez Gene ID: (Human) 10524, (Mouse) 81601, (Rat) 192218
transcription coactivator activity
histone acetyltransferase activity
protein binding
acetyltransferase activity
metal ion binding
androgen receptor binding
repressing transcription factor binding
chromatin binding
transcription factor binding
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
protein complex binding
phospholipase binding
histone modifying enzyme
negative regulation of transcription from RNA polymerase II promoter
DNA double-strand break processing
DNA synthesis involved in DNA repair
strand displacement
DNA replication
double-strand break repair
double-strand break repair via nonhomologous end joining
transcription, DNA-templated
DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
response to ionizing radiation
viral process
histone acetylation
androgen receptor signaling pathway
negative regulation of interleukin-2 production
regulation of growth
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to estradiol stimulus
regulation of signal transduction by p53 class mediator
positive regulation of protein acetylation
beta-catenin-TCF complex assembly
regulation of transcription, DNA-templated
chromatin modification
apoptotic process
response to heat
histone H4 acetylation
cellular response to hydrogen peroxide
cellular response to X-ray
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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