|Tested species reactivity||Human, Mouse, Rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide DDKKHHDAEHHRDREPGT conjugated to BSA, corresponding to amino acids 181-198 of Human TLE1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2, with 50% ammonium sulfate|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:200-1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:1000|
|Western Blot (WB)||1:200 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28063 detects TLE1 from mouse, human, Xenopus laevis and rat samples. Expected to cross-react with chicken (92% identity with immunogen) due to sequence homology.
PA1-28063 has been successfully used in immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), and Western blot applications. By Western Blot a band of ~83.5 kDa is detected.
The PA1-28063 immunogen is Synthetic peptide: DDKKHHDAEHHRDREPGT conjugated to BSA, corresponding to amino acids 181-198 of Human TLE1.
Store at 4°C short term. For extended storage, store at -20°C, avoiding freeze/thaw cycles.
Groucho (TLE1) (Transduction-like enhancer protein 1, ESG1) is a member of the Groucho family of transcriptional corepressors and is involved in oligomerization, association with chromatin and interaction with histone H3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.