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Invitrogen
TLK2 Polyclonal Antibody
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FIGURE: 1 / 2
TLK2 Antibody (PA5-93211) in WB
Western blot analysis of TLK2 in extracts of various cell lines. Samples were incubated with TLK2 Polyclonal antibody (Product # PA5-93211) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-93211
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLK2 (NP_0068432)
Conjugate
Form
Liquid
Concentration
0.44 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MMEELHSLDP RRQELLEARF TGVGVSKGPL NSESSNQSLC SVGSLSDKEV ETPEKKQNDQ RNRKRKAEPY ETSQGKGTPR GHKISDYFEF AGGSAPGTSP GRSVPPVARS SPQHSLSNPL PRRVEQPLYG LDGSAAKEAT EEQSALPTLM SVMLAKPRLD TEQLAQRGAG LCFTFVSAQQ NSPSSTGSGN TEHSCSSQKQ ISIQHRQTQS DLTIEKISAL; Positive Samples: 293T, Mouse testis; Cellular Location: Cytoplasm, Nucleus, cytoskeleton, perinuclear region
Target Information
TLK2 or Tousled-like kinase 2, is a nuclear serine/threonine kinases that is thought to be involved in the regulation of chromatin assembly. In addition, TLK2 is also involved in other cellular processes such as intracellular signaling cascade, protein amino acid phosphorylation, cell cycle, chromatin modification and in response to DNA damage stimulus. TLK2 displays maximal activity during S phase of the cell cycle where it appears to be regulated by cell-cycle-dependent phosphorylation. Inhibition of DNA replication causes a rapid loss of TLK2 activity, indicating that TLK2 function is tightly linked to ongoing DNA replication. The TLK2 enzyme is cell-cycle regulated, with maximal activity in S-phase. It is inactivated by phosphorylation at Ser-750, potentially by CHK1. TLK2 heterodimerizes with TLK1. This nuclear protein is widely expressed, with presence in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle tissues, and in several adult cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HsHPK; PKU-alpha; protein kinase U-alpha; Serine/threonine-protein kinase tousled-like 2; Tousled-like kinase 2
Gene Aliases: 4933403M19Rik; HsHPK; PKU-ALPHA; PKUalpha; Tlk; TLK2
UniProt ID: (Human) Q86UE8, (Mouse) O55047
Entrez Gene ID: (Human) 11011, (Mouse) 24086
regulation of chromatin assembly or disassembly
protein phosphorylation
cellular response to DNA damage stimulus
cell cycle
chromosome segregation
negative regulation of autophagy
covalent chromatin modification
peptidyl-serine phosphorylation
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
intracellular signal transduction
cellular response to gamma radiation
multicellular organism development
spermatogenesis
phosphorylation
chromatin modification
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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