Western blot analysis of TLR11 in either mouse testis lysate in A) the absence and B) presence of blocking peptide or rat testis lysate in C) the absence and D) presence of blocking peptide using a TLR11 polyclonal antibody (Product # PA1-41080) at a 3 ug/ml concentration.
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 911-926 (CEQLKRRLSKAGQERD) of mouse TLR11|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5-1 µg/10^6 cells|
|Western Blot (WB)||1-3 µg/ml|
Toll like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR11 is one of three mouse TLRs that lack a human ortholog. It is activated specifically by uropathogenic bacteria, and mice lacking TLR11 showed a much greater susceptability to uropathogenic infections, indicating a potentially important role for TLR11 in preventing infections in the urogenital system.