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Invitrogen
TLR5 Polyclonal Antibody
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FIGURE: 1 / 4
TLR5 Antibody (PA5-95257) in IHC (P)
Immunohistochemistry analysis of TLR5 in paraffin-embedded human appendicitis tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with TLR5 polyclonal antibody (Product # PA5-95257) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and... View More
Product Details
PA5-95257
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TLR5 (801-828aa MKHQSIRGFVQKQQYLRWPEDFQDVGWF).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: JURKAT whole cell. IHC: human appendicitis tissue. Flow: Human PBMC cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
TLR5 is a Type I membrane protein belonging to the Toll-like receptor family, participates in the innate immune response to microbial agents. TLR5 also plays a role in mediating detection of bacterial flagellins. TLR5 acts via MyD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. TLR5 protein binds to TIRAP and MyD88 via their respective TIR domains TLR5 is highly expressed in liver, and is detected in lung and at very low levels in most other tissues. The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella. Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA). The strain MOLF/Ei is highly susceptible to the infection, has a unique TLR5 haplotype and a lower expression of TRL5. A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires' disease and demonstrates the importance of TLR5 in the innate immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ10052; MGC126430; MGC126431; RP11-239E10.1; systemic lupus erythematosus susceptibility 1; Toll-like receptor 5; Toll/interleukin-1 receptor-like protein 3
Gene Aliases: MELIOS; SLE1; SLEB1; TIL3; TLR5
UniProt ID: (Human) O60602
Entrez Gene ID: (Human) 7100
MyD88-dependent toll-like receptor signaling pathway
inflammatory response
male gonad development
positive regulation of interleukin-8 production
positive regulation of toll-like receptor signaling pathway
toll-like receptor 5 signaling pathway
innate immune response
positive regulation of nitric oxide biosynthetic process
regulation of cytokine secretion
defense response to Gram-negative bacterium
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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