|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues Y(465)FRYDKYARSCRFKNKEAS(483) of human TLR7.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-20817 detects TLR7 in human and mouse samples.
PA1-20817 has been successfully used in immunohistochemistry (paraffin) and Western Blot procedures.
The PA1-20817 immunogen ynthetic peptide, conjugated to KLH, corresponding to residues Y(465)FRYDKYARSCRFKNKEAS(483) of human TLR7. This sequence is 100% conserved in mouse and rat TLR7.
The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL 1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1 10) have been described. Stimulation of the NFkB signaling pathway by TLR7 suggests that it plays a role in immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Molecular Determinants of GS-9620-Dependent TLR7 Activation.
PA1-20817 was used in western blot to characterize GS-9620-dependent TLR7 activation and the associated molecular determinants
|Rebbapragada I,Birkus G,Perry J,Xing W,Kwon H,Pflanz S||PloS one (11:null)||2016|