Immunofluorescent analysis of TLR7 in TLR7 (A) and glial fibrillary acidic protein (GFAP) (B) in murine myenteric plexus using a TLR7 polyclonal antibody (Product # PA1-28109). Most of the GFAP positive cells were also positive for TLR7 in the merged image (C, yellow staining).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues L(706) S H N Q L T T V P E R L S N C S R S L K N L(728) of human TLR7.|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2-5 µg for 10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28109 detects TLR7 from human samples. PA1-28109 is expected to cross-react with rat and mouse due to sequence homology (86% identity with immunogen for both species).
PA1-28109 has been successfully used in immunohistochemistry (frozen tissue), flow cytometry and Western blot applications.
The PA1-28109 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues L(706) S H N Q L T T V P E R L S N C S R S L K N L(728) of human TLR7.
The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL 1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TRLs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Ten human homolgs of TLRs (TLR1 10) have ben described. Stimulation of the NFkB signaling pathway by TLR7 suggest that it plays a role in immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.