Intracellular flow analysis of TLR7 in 10^6 ThP1 cells using 2 ug of TLR7 polyclonal antibody (Product # PA5-23488). Shaded histogram represents cells without antibody; green represents isotype control; red represents TLR7 polyclonal antibody (Product # PA5-23488). An anti-rabbit IgG PE conjugated secondary.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (aa 684-700) of TLR7 protein.|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5-2 µg/1x10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||1:20|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for TLR7 (transient overexpression lysate), Ramos.
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). Stimulation of the NFkB signaling pathway by TLR7 suggests that it plays a role in immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.