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Invitrogen
TMEM119 Polyclonal Antibody
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FIGURE: 1 / 6
TMEM119 Antibody (PA5-119902) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of TMEM119 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with TMEM119 Polyclonal Antibody (Product # PA5-119902) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain i... View More
Product Details
PA5-119902
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within human TMEM119 aa 11-52.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Mouse brain tissue lysate, mouse cerebellum tissue lysate, SH-SY5Y.
Subcellular Location: Endoplasmic reticulum membrane, Cell membrane, Cytoplasm.
Target Information
Tmem119 encodes a single-pass type I transmembrane protein which seems to interact with a multitude of micro-RNAs as well as certain transcription factors such as SMAD1, SMAD5 and RUNX2. Tmem119 has been identified as a surface marker of microglia that can be used to reliably distinguish microglia from infiltrating macrophages. Microglial cells are phagocytes that are restricted to the brain and involved in clearing up debris and dendrite pruning of neurons. They display a high functional plasticity, balancing neuroprotective and neurotoxic functions, which is reflected by their distinct physiology in response to changes in their microenvironment. Microglia are particularly mobile in areas of the brain undergoing restructuring. Reactive microglia co-express the marker Iba1. In a pathological context, microglia have been associated with differentiation of pathogenic/inflammatory A1 type astrocytes by secretion of Cq1, IL-1a and TNF when stimulated with LPS, and thought to contribute to the pathology of several neurodegenerative diseases such as Alzheimers, Parkinsons, Chorea Huntingtons disease and Multiple Sclerosis. The specific function Tmem119 plays on microglial cells remains unclear. Outside of the neural system, Tmem119 seems to be involved in osteoblast differentiation/ossification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: OBIF; Osteoblast induction factor; Transmembrane protein 119; unnamed protein product
Gene Aliases: AW208946; BC025600; OBIF; PSEC0199; TMEM119; UNQ731/PRO1415
UniProt ID: (Human) Q4V9L6, (Mouse) Q8R138
Entrez Gene ID: (Human) 338773, (Mouse) 231633
ossification
osteoblast differentiation
endochondral ossification
spermatogenesis
positive regulation of gene expression
negative regulation of myotube differentiation
cell differentiation
positive regulation of bone mineralization
BMP signaling pathway
biomineral tissue development
positive regulation of osteoblast proliferation
positive regulation of osteoblast differentiation
negative regulation of bone resorption
spermatid differentiation
positive regulation of bone development
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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