Immunofluorescent analysis of TMF1 showing staining in the Golgi apparatus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a TMF1 polyclonal antibody (Product # PA5-31184) diluted at 1:5000. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 172 and 422 of Human TMF1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31184 targets TMF1 in IHC (P) and WB applications and shows reactivity with Human and Rat samples.
The PA5-31184 immunogen is recombinant fragment corresponding to a region within amino acids 172 and 422 of Human TMF1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.