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Invitrogen
TMSB4X Polyclonal Antibody
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FIGURE: 1 / 3
TMSB4X Antibody (PA5-47875) in ICC/IF
Immunocytochemistry analysis of TMSB4X in immersion fixed HeLa human cervical epithelial carcinoma cell line. Samples were incubated in TMSB4X polyclonal antibody (Product # PA5-47875) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm.
Product Details
PA5-47875
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human Thymosin beta 4 Ser2-Ser44
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Thymosin beta 10 and rhThymosin beta 16 is observed.
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Target Information
Thymosin-beta4 is a small, actin-sequestering protein belonging to the thymosin-beta family that is found at high concentrations within the spleen, thymus, and peritoneal macrophages, where it is most notably responsible for the organization of cytoskeletal structure. In mammalian tissues, this protein acts as a modulator for the polymerization/depolymerization of actin through the formation of a 1:1 complex with the monomer G (globular)-actin, and inhibits actin's polymerization to form F (filamentous) actin, which together with other proteins binds microfilaments to construct the cytoskeleton. Commonly found at significant quantities within the brain, lungs, liver, kidneys, testes, and heart, thymosin-beta4 has also been shown to be synthesized by cells unrelated to the reticuloendothelial system, such as myoblasts and fibroblasts, and expressed at irregular levels by several hemopoietic cell lines, malignant lymphoid cells and myeloma cells. In addition to regulating actin polymerization, research has also found thymosin-beta4 to stimulate the secretion of hypothalamic luteinizing hormone-releasing hormone and luteinizing hormone, inhibit the migration of peritoneal macrophages, induce phenotypic changes in T cell lines during early host defense mechanisms, and inhibit the progression of hematopoietic pluripotent stem cells into the S-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Fx; prothymosin beta-4; T beta-4; TB4Xbeta 4; thymosin; thymosin beta 4; Thymosin beta-4; thymosin, beta 4, X chromosome; unnamed protein product
Gene Aliases: FX; PTMB4; TB4X; THYB4; TMSB4; TMSB4X
UniProt ID: (Human) P62328
Entrez Gene ID: (Human) 7114
actin filament organization
regulation of cell migration
negative regulation of actin filament polymerization
tumor necrosis factor-mediated signaling pathway
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of blood vessel endothelial cell migration
negative regulation of inflammatory response
negative regulation of hematopoietic stem cell differentiation
negative regulation of cell cycle G1/S phase transition
positive regulation of proton-transporting ATP synthase activity, rotational mechanism
positive regulation of endothelial cell chemotaxis
positive regulation of ATP biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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