|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant rat TNF alpha|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PRTNFAI targets TNF Alpha in ELISA, and WB applications and shows reactivity with Rat samples.
The PRTNFAI immunogen is recombinant rat TNF alpha.
PRTNFAI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa.
The PRTNFAI rat TNFa polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with polyclonal biotinylated detection antibody PRTNFAI. Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protective effects of Lactobacillus paracasei F19 in a rat model of oxidative and metabolic hepatic injury.
PRTNFAI was used in western blot to investigate the protective effect of Lactobacillus paracasei F19 against liver injury in rats
|Nardone G,Compare D,Liguori E,Di Mauro V,Rocco A,Barone M,Napoli A,Lapi D,Iovene MR,Colantuoni A||American journal of physiology. Gastrointestinal and liver physiology (299:G669)||2010|
Expression of hypothalamic neuropeptides and the desensitization of pituitary-adrenal axis and hypophagia in the endotoxin tolerance.
PRTNFAI was used in ELISA to investigate the hypothalamic neuropeptides and their involvement in the endotoxin tolerance
|Borges BC,Antunes-Rodrigues J,Castro M,Bittencourt JC,Elias CF,Elias LL||Hormones and behavior (52:508)||2007|