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Invitrogen
TNF alpha Polyclonal Antibody, Biotin, PeproTech®
FIGURE: 1 / 3
TNF alpha Antibody (500-P64BT-1MG) in WB
Western Blot: To detect Murine TNF-alpha by Western Blot analysis TNF alpha Polyclonal Antibody, Biotin (Product # 500-P64BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine TNF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P64BT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Published species
Bacteria,
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 17.3 kDa Recombinant Murine TNF-alpha
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930674
Product Specific Information
AA Sequence of recombinant protein: MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine TNF-alpha. Anti-Murine TNF-alpha-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine TNF-alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine TNF-alpha (500-P64) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine TNF-alpha.
Western Blot: To detect Murine TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine TNF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P64BT-1MG will be provided as 2 x 500 µg
Target Information
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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