|Tested species reactivity||Rat|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified recombinant rat TNF-á.|
|Storage buffer||PBS, pH 7.3|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Neuroprotective Effects of Direct Intrathecal Administration of Granulocyte Colony-Stimulating Factor in Rats with Spinal Cord Injury.
ARC3012 was used in immunohistochemistry to investigate the therapeutic efficacy of intrathecal administration of granulocyte colony-stimulating factor in spinal cord injuries
|Chen WF,Chen CH,Chen NF,Sung CS,Wen ZH||CNS neuroscience and therapeutics (21:698)||2015|
Involvement of phosphatase and tensin homolog deleted from chromosome 10 in rodent model of neuropathic pain.
ARC3012 was used in immunohistochemistry - frozen section to study PTEN in a rodent model of neuropathic pain
|Huang SY,Sung CS,Chen WF,Chen CH,Feng CW,Yang SN,Hung HC,Chen NF,Lin PR,Chen SC,Wang HM,Chu TH,Tai MH,Wen ZH||Journal of neuroinflammation (12:null)||2015|
Peripheral noxious stimulation reduces withdrawal threshold to mechanical stimuli after spinal cord injury: role of tumor necrosis factor alpha and apoptosis.
ARC3012 was used in western blot to test if noxious input affects the development and maintenance of chronic neuropathic pain
|Garraway SM,Woller SA,Huie JR,Hartman JJ,Hook MA,Miranda RC,Huang YJ,Ferguson AR,Grau JW||Pain (155:2344)||2014|
Insulin receptor and IRS-1 co-immunoprecipitation with SOCS-3, and IKK¿/ß phosphorylation are increased in obese Zucker rat skeletal muscle.
ARC3012 was used in western blot to study factors that promote insulin-stimulated phosphatidylinositol 3-kinase activity in the skeletal muscle of obese Zucker rats.
|Zolotnik IA,Figueroa TY,Yaspelkis BB||Life sciences (91:816)||2012|
|Mouse||Not Cited||Functional link between TNF biosynthesis and CaM-dependent activation of inducible nitric oxide synthase in RAW 264.7 macrophages.||Weber TJ,Smallwood HS,Kathmann LE,Markillie LM,Squier TC,Thrall BD||American journal of physiology. Cell physiology (290:C1512)||2006|
TGF-ß1 attenuates spinal neuroinflammation and the excitatory amino acid system in rats with neuropathic pain.
ARC3012 was used in immunohistochemistry (frozen) to study the antinociceptive effects of TGF-β1 on chronic constriction injury-induced neuropathic pain in rats.
|Chen NF,Huang SY,Chen WF,Chen CH,Lu CH,Chen CL,Yang SN,Wang HM,Wen ZH||The journal of pain : official journal of the American Pain Society (14:1671)||2013|
Aerobic training reverses high-fat diet-induced pro-inflammatory signalling in rat skeletal muscle.
ARC3012 was used in immunoprecipitation and western blot to test if aerobic training reverses PI-3 kinase activation
|Yaspelkis BB,Kvasha IA,Lessard SJ,Rivas DA,Hawley JA||European journal of applied physiology (110:779)||2010|
|Not Applicable||Not Cited||
High-fat feeding increases insulin receptor and IRS-1 coimmunoprecipitation with SOCS-3, IKKalpha/beta phosphorylation and decreases PI-3 kinase activity in muscle.
ARC3012 was used in immunoprecipitation and western blot to elucidate the role of SOCS-3 in skeletal muscle insulin resistance
|Yaspelkis BB,Kvasha IA,Figueroa TY||American journal of physiology. Regulatory, integrative and comparative physiology (296:R1709)||2009|